AF 488 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11820 | 1 mg | $125 | in stock | |
| 21820 | 5 mg | $325 | in stock | |
| 31820 | 10 mg |
$480
|
in stock | |
| 41820 | 25 mg |
$950
|
in stock | |
| 51820 | 50 mg |
$1625
|
in stock | |
| 61820 | 100 mg |
$2090
|
in stock |
AF 488 is a bright and photostable dye. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.
AF 488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated, otherwise, the use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF 488.
This NHS ester is an amine-reactive dye; it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.
Absorption and emission spectra of AF 488
Customers also purchased with this product
Cyanine3B maleimide
Derivative of a Cyanine3B dye, improved Cyanine3 dye to have higher quantum yield, for maleimide reaction chemistry.
Add this product to your cart andget free express delivery
Annexin V-AF 488 conjugate
Annexin V-AF 488 conjugate for detection of apoptotic cells by flow cytometry and fluorescence microscopy.Cyanine7 DBCO
NIR fluorescent dye functionalized with cyclooctyne moiety for copper free click chemistry conjugation with azides.
Add this product to your cart andget free express delivery
General properties
| Appearance: | dark orange solid |
| Molecular weight: | 732.74 |
| Molecular formula: | C31H32N4O13S2 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (80+%, balance mostly carboxylic acid) |
| Storage conditions: | Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
Product citations
- Schöpf, F.; Marongiu, G.L.; Milaj, K.; Sprink, T.; Kikhney, J.; Moter, A.; Roderer, D. Structural basis of Fusobacterium nucleatumadhesin Fap2 interaction with receptors on cancer and immune cells. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.28.582045
- Białobrzewski, M.K.; Klepka, B.P.; Michaś, A.; Cieplak-Rotowska, M.K.; Staszałek, Z.; Niedźwiecka, A. Diversity of hydrodynamic radii of intrinsically disordered proteins. European Biophysics Journal, 2023, 52, 607-618. doi: 10.1007/s00249-023-01683-8
- Makri Pistikou, A.-M.; Cremers, G.A.O.; Nathalia, B.L.; Meuleman, T.J.; Bögels, B.W.A.; Eijkens, B.V.; de Dreu, A.; Bezembinder, M.T.H.; Stassen, O.M.J.A.; Bouten, C.C.V.; Merkx, M.; Jerala, R.; de Greef, T.F.A. Engineering a scalable and orthogonal platform for synthetic communication in mammalian cells. Nature Communications, 2023, 14, 7001. doi: 10.1038/s41467-023-42810-5
- Wiest, M.J.; Baert, L.; Gu, C.; Gayler, K.M.; Ham, H.; Gorvel, L.; Keddis, M.T.; Griffing, L.W.; Joo, H.M.; Gorvel, J.-P.; Billadeau, D.D.; Kane, R.R.; Oh, S.K. Endosomal trafficking inhibitor EGA can control TLR7-mediated IFNα expression by human plasmacytoid dendritic cells. Frontiers in Immunology, 2023, 14, 1202197. doi: 10.3389/fimmu.2023.1202197
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
The count of items is incorrect.
Your cart is empty
$ 
