Phosphate CPG 500 v2
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17860 | 100 mg | $40 | in stock | |
| 47860 | 1 g | $170 | in stock |
Phosphate CPG 500 v2 allows direct preparation of oligonucleotides with a 3’-phosphate group.
The sulfone linker is one of the linkers employed for the preparation of oligonucleotides, it releases the oligonucleotide 3’-phosphate by β-elimination upon ammonolysis.
Available in different pore sizes, Phosphate CPG 500 v2 with pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 30 bases.
For synthesis longer oligonucleotides 1000 Å CPG can be used.
Usage
Coupling: identical to normal protected nucleoside support.
Deprotection: identical to protected nucleobases.
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dsGreen for staining dsDNA in agarose and polyacrylamide gels.General properties
| Appearance: | slightly yellow beads |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50-80 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | identical to protected nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
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