Custom dual-labeled probe synthesis

The service of custom labeled oligonucleotide and dual-labeled probe synthesis is provided by Lumiprobe.

The probes are synthesized from dyes produced by Lumiprobe (or commercial dyes) using automated phosphoramidite chemistry. The purification is performed by PAGE and subsequent HPLC. This ensures optimal probe quality, highest purity and reproducibility.

Most fluorescent dyes are attached to the DNA using our Click-chemistry based technology. Some are introduced using Lumiprobe or other commercially available phosphoramidites.

Modification availability

Modification	Available labeling site
Modification	5'-terminus	3'-terminus
Fluorescent dyes
FAM
HEX
JOE
TAMRA
R6G
R110
ROX
Cy3
Cy3.5
Cy5
Cy5.5
Cy7
Cy7.5
Fluorescence quenchers
Dabcyl
Other groups
Acetylene
Aminolink
Biotin

*FAM, HEX, JOE, TAMRA, R6G, R110 and ROX are either pure 5- or pure 6-isomers.

*Please note that shipping cost of $50 applies to orders below $400.

Pricing

The prices do not depend on the dyes you have chosen.

Oligo type	nmols guaranteed / price (USD)
Oligo type	3	20	50	100	200	500
Oligonucleotide, 30 bases or below, with HPLC/PAGE purification	N/A	$30	$60	$90	$150	$380
Oligonucleotide, 31-80 bases, with HPLC/PAGE purification	N/A	$40	$85	$125	$210	$530
Oligonucleotide with one modification, 30 bases or below, with HPLC/PAGE purification	$120	$150	$260	Please inquire
Oligonucleotide with one modification, 31-80 bases, with HPLC/PAGE purification	$170	$210	$360
Oligonucleotide with two modifications, 30 bases or below, with HPLC+PAGE purification	$220	$280	$440
Oligonucleotide with two modifications, 31-80 bases, with HPLC+PAGE purification	$310	$390	$610

The prices are in US Dollars. World-wide flat rate shipping cost of $50 applies to orders below $400. Payment is accepted by credit card or wire transfer.