Activated esters

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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Cat. # Quantity Price Lead time
2934-1mg 1 mg –   in stock
2934-25mg 25 mg 160$ in stock
2934-50mg 50 mg 270$ in stock
2934-100mg 100 mg 520$ in stock
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AF 343 (Coumarin)-X-NHS ester

AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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Cat. # Quantity Price Lead time
12620 1 mg 110$ in stock
22620 5 mg 210$ in stock
42620 25 mg 410$ in stock
52620 50 mg 695$ in stock
62620 100 mg 1190$ in stock
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AF 430 NHS ester

AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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Cat. # Quantity Price Lead time
10820 1 mg 110$ in stock
20820 5 mg 165$ in stock
40820 25 mg 380$ in stock
50820 50 mg 695$ in stock
60820 100 mg 1190$ in stock
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AF 488 NHS ester

AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.
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Cat. # Quantity Price Lead time
11820 1 mg 125$ in stock
21820 5 mg 325$ in stock
31820 10 mg 480$ in stock
41820 25 mg 950$ in stock
51820 50 mg 1625$ in stock
61820 100 mg 2090$ in stock
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AF 488 TFP ester

Tetrafluorophenyl ester of green fluorescent dye AF 488 for labeling amino groups in proteins, peptides, and modified oligonucleotides.
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Cat. # Quantity Price Lead time
118T0 1 mg 165$ 21 days
218T0 5 mg 510$ 6 days
418T0 25 mg 1650$ 6 days
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AF 532 NHS ester

NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.
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Cat. # Quantity Price Lead time
1C820 1 mg 140$ in stock
2C820 5 mg 450$ in stock
4C820 25 mg 1460$ in stock
5C820 50 mg 1890$ in stock
6C820 100 mg 2640$ in stock
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AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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Cat. # Quantity Price Lead time
1K820 1 mg 140$ in stock
2K820 5 mg 450$ in stock
4K820 25 mg 1460$ in stock
5K820 50 mg 1890$ in stock
6K820 100 mg 2640$ in stock
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AF 568 NHS ester

AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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Cat. # Quantity Price Lead time
14820 1 mg 125$ in stock
24820 5 mg 325$ in stock
44820 25 mg 720$ in stock
54820 50 mg 1325$ in stock
64820 100 mg 2090$ in stock
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AF 594 NHS ester

Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomer
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Cat. # Quantity Price Lead time
2825-1mg 1 mg 125$ in stock
2825-5mg 5 mg 425$ in stock
2825-25mg 25 mg 1240$ in stock
2825-50mg 50 mg 1890$ in stock
2825-100mg 100 mg 2640$ in stock
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AF 647 NHS ester

Bright, water-soluble succinimide ester of AF 647 dye for protein and antibody labeling.
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Cat. # Quantity Price Lead time
16820 1 mg 140$ in stock
26820 5 mg 450$ 5 days
46820 25 mg 1460$ in stock
56820 50 mg 1890$ in stock
66820 100 mg 2640$ in stock
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AMCA NHS ester

NHS-ester of AMCA dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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Cat. # Quantity Price Lead time
3606-5mg 5 mg 110$ in stock
3606-25mg 25 mg 290$ in stock
3606-50mg 50 mg 435$ in stock
3606-100mg 100 mg 595$ in stock
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ATT 550 NHS ester

Water-soluble ATT 550 succinimidyl ester for labeling various amine-containing molecules in the aqueous phase.
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Cat. # Quantity Price Lead time
1C920 1 mg 105$ in stock
2C920 5 mg 628$ in stock
4C920 25 mg 1620$ in stock
5C920 50 mg 2040$ in stock
6C920 100 mg 2790$ in stock
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BDP® 558/568 NHS ester

BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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Cat. # Quantity Price Lead time
17420 1 mg 125$ in stock
27420 5 mg 260$ in stock
47420 25 mg 510$ in stock
57420 50 mg 895$ in stock
67420 100 mg 1490$ in stock
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BDP 564/570 NHS ester

Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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Cat. # Quantity Price Lead time
1H420 1 mg 125$ in stock
2H420 5 mg 325$ in stock
4H420 25 mg 510$ in stock
5H420 50 mg 895$ in stock
6H420 100 mg 1490$ in stock
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BDP® 576/589 NHS ester

BDP 576/589 NHS ester is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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Cat. # Quantity Price Lead time
1G420 1 mg 125$ in stock
2G420 5 mg 260$ in stock
4G420 25 mg 510$ in stock
5G420 50 mg 895$ in stock
6G420 100 mg 1490$ in stock
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BDP® 581/591 NHS ester

NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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Cat. # Quantity Price Lead time
16420 1 mg 125$ in stock
26420 5 mg 260$ in stock
46420 25 mg 510$ in stock
56420 50 mg 895$ in stock
66420 100 mg 1490$ in stock
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BDP® 630/650-X-NHS ester

BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays. This is an amine-reactive NHS ester derivative.
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Cat. # Quantity Price Lead time
15420 1 mg 125$ in stock
25420 5 mg 260$ in stock
45420 25 mg 510$ in stock
55420 50 mg 895$ in stock
65420 100 mg 1490$ in stock
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BDP® 650/665-X-NHS ester

Amine-reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel possessing a high molar extinction coefficient and a good emission quantum yield.
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Cat. # Quantity Price Lead time
1E420 1 mg 125$ in stock
2E420 5 mg 260$ in stock
4E420 25 mg 510$ in stock
5E420 50 mg 895$ in stock
6E420 100 mg 1490$ in stock
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BDPep 630/650 NHS ester

Dicyano BDP 630/650 is a far-red fluorescent dye from the boron dipyrromethene (BDP) family, known for its high brightness and exceptional photostability. Its remarkable stability in strong acidic TFA media makes it particularly suitable for solid-phase peptide synthesis (SPPS). BDPep 630/650 NHS ester can be used for effective cell sorting and phenotyping.
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Cat. # Quantity Price Lead time
1M420 1 mg 125$ in stock
2M420 5 mg 260$ in stock
4M420 25 mg 510$ 6 days
5M420 50 mg 895$ 21 days
6M420 100 mg 1490$ 21 days
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BDPep FL NHS ester

BDP FL analog derivative suitable for solid phase synthesis SPPS.
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Cat. # Quantity Price Lead time
1J420 1 mg –   in stock
2J420 5 mg 260$ in stock
4J420 25 mg 510$ in stock
5J420 50 mg 895$ in stock
6J420 100 mg 1490$ in stock
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BDP® FL NHS ester

Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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Cat. # Quantity Price Lead time
11420 1 mg 125$ 98$ in stock
21420 5 mg 260$ 175$ in stock
41420 25 mg 485$ in stock
51420 50 mg 824$ in stock
61420 100 mg 1380$ in stock
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BDP® R6G NHS ester

BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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Cat. # Quantity Price Lead time
14420 1 mg 125$ in stock
24420 5 mg 260$ in stock
44420 25 mg 510$ in stock
54420 50 mg 895$ in stock
64420 100 mg 1490$ in stock
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BDP® TMR NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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Cat. # Quantity Price Lead time
12420 1 mg 125$ in stock
22420 5 mg 260$ in stock
42420 25 mg 510$ in stock
52420 50 mg 895$ in stock
62420 100 mg 1490$ in stock
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BDP® TMR-X-NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays. This compound contains an aminohexanoic acid (C6) linker between the dye and the functional group.
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Cat. # Quantity Price Lead time
1F420 1 mg 125$ in stock
2F420 5 mg 260$ in stock
4F420 25 mg 510$ in stock
5F420 50 mg 895$ in stock
6F420 100 mg 1490$ in stock
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BDP® TR-X-NHS ester

BDP TR-X-NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used to conjugate proteins and peptides.
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Cat. # Quantity Price Lead time
1D420 1 mg 125$ in stock
2D420 5 mg 325$ in stock
4D420 25 mg 510$ in stock
5D420 50 mg 895$ in stock
6D420 100 mg 1490$ in stock
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Cyanine3.5 NHS ester

Cyanine3.5 dye NHS ester derivative the labeling of amino-groups in biomolecules.
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Cat. # Quantity Price Lead time
12020 1 mg 125$ in stock
22020 5 mg 260$ in stock
42020 25 mg 510$ in stock
52020 50 mg 895$ in stock
62020 100 mg 1490$ in stock
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Cyanine3B NHS ester

Cyanine3B NHS ester is a reactive dye with significantly increased fluorescence quantum yield and photostability for the specific labeling of amino-groups in biomolecules.
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Cat. # Quantity Price Lead time
19320 1 mg 140$ in stock
29320 5 mg 340$ in stock
49320 25 mg 990$ in stock
59320 50 mg 1690$ in stock
69320 100 mg 3100$ in stock
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Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester for the labeling of amino-groups.
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Cat. # Quantity Price Lead time
11020 1 mg 89$ in stock
21020 5 mg 115$ in stock
41020 25 mg 375$ in stock
51020 50 mg 665$ in stock
61020 100 mg 1490$ in stock
81020 1 g please inquire 6 days
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Cyanine5.5 NHS ester

Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.
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Cat. # Quantity Price Lead time
17020 1 mg 125$ in stock
27020 5 mg 260$ in stock
47020 25 mg 510$ in stock
57020 50 mg 895$ in stock
67020 100 mg 1490$ in stock
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Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.
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Cat. # Quantity Price Lead time
13020 1 mg 85$ in stock
23020 5 mg 115$ in stock
43020 25 mg 335$ in stock
53020 50 mg 545$ in stock
63020 100 mg 865$ in stock
83020 1 g please inquire 10 days
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Cyanine7.5 NHS ester

Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5 .
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Cat. # Quantity Price Lead time
16020 1 mg 125$ in stock
26020 5 mg 260$ in stock
46020 25 mg 510$ in stock
56020 50 mg 895$ in stock
66020 100 mg 1490$ in stock
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Cyanine7 NHS ester

Amine reactive NHS ester of near infrared fluorescent dye, an improved analog of Cy7®.
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Cat. # Quantity Price Lead time
15020 1 mg 125$ in stock
25020 5 mg 260$ in stock
45020 25 mg 510$ in stock
55020 50 mg 895$ in stock
65020 100 mg 1490$ in stock
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DusQ 1 NHS ester

Functionalized DusQ 1 quencher derivative with quenching range from 480 - 580 nm for labeling of amine-containing molecules.
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Cat. # Quantity Price Lead time
21D20 5 mg –   in stock
41D20 25 mg 315$ in stock
51D20 50 mg 580$ in stock
61D20 100 mg 885$ in stock
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DusQ 21 NHS ester

Amino-reactive NHS ester with quencher for the conjugation with amines-containing molecules, DusQ 21 quenching range 590 - 720 nm.
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Cat. # Quantity Price Lead time
28D20 5 mg –   in stock
48D20 25 mg 315$ in stock
68D20 100 mg 885$ in stock
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DusQ 2 NHS ester

DusQ 2 dark quencher with a quenching range from 560 - 670 nm with primary amine reactivity for synthesis of FRET probes. This NHS ester can be coupled to various molecules bearing amino groups.
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Cat. # Quantity Price Lead time
22D20 5 mg –   in stock
42D20 25 mg 315$ in stock
52D20 50 mg 580$ in stock
62D20 100 mg 885$ in stock
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DusQ 3 NHS ester

Amino-reactive NHS ester for the labeling with far-red quencher DusQ 3.
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Cat. # Quantity Price Lead time
23D20 5 mg –   in stock
43D20 25 mg 315$ in stock
53D20 50 mg 580$ in stock
63D20 100 mg 885$ in stock
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FAM isothiocyanate (FITC), 5- and 6-isomers

An isothiocyanate derivative of fluorescein for labeling of antibodies and other biomolecules. Mix of 5- and 6-isomers.
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Cat. # Quantity Price Lead time
3524-100mg 100 mg 43$ in stock
3524-500mg 500 mg 249$ 147$ in stock
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FAM NHS ester, 6-isomer

Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.
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Cat. # Quantity Price Lead time
15120 1 mg –   in stock
25120 5 mg 110$ in stock
45120 25 mg 145$ in stock
55120 50 mg 260$ in stock
65120 100 mg 470$ in stock
85120 1 g please inquire in stock
95120 5 g please inquire in stock
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HEX NHS ester, 6-isomer

NHS-ester of HEX dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups.
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Cat. # Quantity Price Lead time
2B220 5 mg 175$ in stock
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JOE NHS ester, 5-isomer

JOE is a fluorescent dye with emission in the yellow part of the spectrum. This derivative is an activated NHS ester for conjugation with amines.
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Cat. # Quantity Price Lead time
11120 1 mg 110$ in stock
21120 5 mg 210$ in stock
41120 25 mg 410$ in stock
51120 50 mg 695$ in stock
61120 100 mg 1190$ in stock
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Pyrenebutyric acid NHS ester

Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.
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Cat. # Quantity Price Lead time
63520 100 mg 110$ in stock
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R6G NHS ester, 5-isomer

R6G (rhodamine 6G) NHS ester, pure 5-isomer. This dye is often used for the labeling of oligonucleotides for qPCR.
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Cat. # Quantity Price Lead time
14220 1 mg 110$ in stock
24220 5 mg 210$ in stock
44220 25 mg 410$ in stock
54220 50 mg 695$ in stock
64220 100 mg 1190$ in stock
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ROX NHS ester, 5-isomer

Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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Cat. # Quantity Price Lead time
11220 1 mg 110$ in stock
21220 5 mg 210$ in stock
41220 25 mg 410$ in stock
51220 50 mg 695$ in stock
61220 100 mg 1190$ in stock
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ROX NHS ester, 6-isomer

Pure 6-isomer of ROX (Rhodamine X, R101) NHS-ester for amine labeling.
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Cat. # Quantity Price Lead time
12220 1 mg 110$ in stock
22220 5 mg 210$ in stock
42220 25 mg 410$ in stock
52220 50 mg 695$ in stock
62220 100 mg 1190$ in stock
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sulfo-Cyanine3.5 NHS ester

Amine-reactive activated ester of water-soluble, sulfonated sulfo-Cyanine3.5 dye.
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Cat. # Quantity Price Lead time
12320 1 mg 125$ in stock
22320 5 mg 325$ in stock
42320 25 mg 850$ in stock
52320 50 mg 1490$ in stock
62320 100 mg 1990$ in stock
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sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.
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Cat. # Quantity Price Lead time
11320 1 mg 89$ in stock
21320 5 mg 115$ in stock
41320 25 mg 390$ in stock
51320 50 mg 725$ in stock
61320 100 mg 1280$ in stock
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sulfo-Cyanine5.5 NHS ester

Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.
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Cat. # Quantity Price Lead time
17320 1 mg 125$ in stock
27320 5 mg 325$ 5 days
47320 25 mg 850$ in stock
57320 50 mg 1490$ 5 days
67320 100 mg 1990$ in stock
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sulfo-Cyanine5 alkyne NHS ester

Bifunctional derivative of sulfo-Cyanine5 dye containing reactive groups of terminal alkyne and NHS ester.
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NO items of this product available in stock but you can submit request for bulk.
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sulfo-Cyanine5 bis-NHS ester

Water soluble amine reactive Cyanine5 dye with two NHS ester groups.
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Cat. # Quantity Price Lead time
133D0 1 mg 125$ in stock
233D0 5 mg 325$ in stock
433D0 25 mg 850$ in stock
533D0 50 mg 1490$ in stock
633D0 100 mg 1990$ in stock
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sulfo-Cyanine5 NHS ester

Water-soluble sulfo-Cyanine5 succinimidyl ester for labeling various amine-containing molecules in the aqueous phase.
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Cat. # Quantity Price Lead time
13320 1 mg 89$ in stock
23320 5 mg 115$ in stock
43320 25 mg 340$ in stock
53320 50 mg 552$ in stock
63320 100 mg 885$ in stock
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sulfo-Cyanine7.5 NHS ester

A water-soluble near-infrared emitting dye for in vivo imaging applications. sulfo-Cyanine7.5 is an analog of indocyanine green (ICG) possessing higher emission quantum yield and a linker arm.
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Cat. # Quantity Price Lead time
16320 1 mg 125$ in stock
26320 5 mg 325$ in stock
46320 25 mg 850$ in stock
56320 50 mg 1490$ in stock
66320 100 mg 1990$ in stock
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sulfo-Cyanine7 bis-NHS ester

sulfo-Cyanine7 bis-NHS ester is a bifunctional derivative of water-soluble near-infrared sulfo-Cyanine7. It contains two activated ester functional groups.
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Cat. # Quantity Price Lead time
153D0 1 mg 125$ in stock
253D0 5 mg 325$ in stock
453D0 25 mg 850$ in stock
553D0 50 mg 1490$ in stock
653D0 100 mg 1990$ in stock
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sulfo-Cyanine7 NHS ester

sulfo-Cyanine7 NHS ester is a water-soluble, near-infrared, amine-reactive dye.
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Cat. # Quantity Price Lead time
15320 1 mg 125$ in stock
25320 5 mg 325$ in stock
45320 25 mg 850$ in stock
55320 50 mg 1490$ in stock
65320 100 mg 1990$ in stock
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TAMRA NHS ester, 5-isomer

Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides. Pure 5-isomer.
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Cat. # Quantity Price Lead time
17120 1 mg –   in stock
27120 5 mg 210$ in stock
47120 25 mg 350$ in stock
57120 50 mg 595$ in stock
67120 100 mg 790$ in stock
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TAMRA NHS ester, 6-isomer

NHS-ester of TAMRA dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups. Pure 6-isomer.
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Cat. # Quantity Price Lead time
18120 1 mg 110$ in stock
28120 5 mg 210$ in stock
48120 25 mg 350$ in stock
58120 50 mg 595$ in stock
68120 100 mg 790$ in stock
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Thiazole Orange NHS ester

Thiazole Orange NHS ester for labeling biomolecules (peptides, proteins, amino-DNA, etc.). Thiazole orange is a cyanine dye that is essentially dark in solution but has a bright fluorescence in double-stranded DNA and RNA.
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Cat. # Quantity Price Lead time
2183-1mg 1 mg –   in stock
2183-5mg 5 mg 60$ in stock
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TR-X-NHS ester, 5-isomer

TR is a red-emitting fluorescent dye based on sulforhodamine scaffold, an amine-reactive NHS ester.
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Cat. # Quantity Price Lead time
1V220 1 mg 125$ in stock
2V220 5 mg 325$ in stock
4V220 25 mg 510$ in stock
5V220 50 mg 895$ in stock
6V220 100 mg 1490$ in stock
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