Cyanine3.5 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12020 | 1 mg | $125 | in stock | |
| 22020 | 5 mg | $260 | in stock | |
| 42020 | 25 mg |
$510
|
in stock | |
| 52020 | 50 mg |
$895
|
in stock | |
| 62020 | 100 mg |
$1490
|
in stock |
Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.
Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, DyLight 594.
Cyanine3.5 NHS ester absorbance and emission spectra
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An activated ester for the introduction of alkyne into peptides/proteins and click reaction.General properties
| Appearance: | dark purple solid |
| Molecular weight: | 741.62 |
| CAS number: | 2231670-85-0 |
| Molecular formula: | C42H44N3BF4O4 |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
| Quality control: | NMR 1H and HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 591 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 604 |
| Fluorescence quantum yield: | 0.35 |
| CF260: | 0.29 |
| CF280: | 0.22 |
Product citations
- Son, T.; Kim, M.; Choi, M.; Nam, S.H.; Yoo, A.; Lee, H.; Han, E.H.; Hong, K.S.; Park, H.S. Advancing fluorescence imaging: enhanced control of cyanine dye-doped silica nanoparticles. Journal of Nanobiotechnology, 2024, 22, 347. doi: 10.1186/s12951-024-02638-7
- Tang, M.; Chen, B.; Xia, H.; Pan, M.; Zhao, R.; Zhou, J.; Yin, Q.; Wan, F.; Yan, Y.; Fu, C.; Zhong, L.; Zhang, Q.; Wang, Y. pH-gated nanoparticles selectively regulate lysosomal function of tumour-associated macrophages for cancer immunotherapy. Nature Communications, 2023, 14, 5888. doi: 10.1038/s41467-023-41592-0
- Yin, Q.; Pan, A.; Chen, B.; Wang, Z.; Tang, M.; Yan, Y.; Wang, Y.; Xia, H.; Chen, W.; Du, H.; Chen, M.; Fu, C.; Wang, Y.; Yuan, X.; Lu, Z.; Zhang, Q.; Wang, Y. Quantitative Imaging of Intracellular Nanoparticle Exposure Enables Prediction of Nanotherapeutic Efficacy. Nat Commun, 2021, 12(1), 2385. doi: 10.1038/s41467-021-22678-z
- Lee, S.A.; Biteen, J.S. Spectral Reshaping of Single Dye Molecules Coupled to Single Plasmonic Nanoparticles. Journal of Physical Chemistry Letters, 2019, 10, 5764–5769. doi: 10.1021/acs.jpclett.9b02480
Cy® is a trademark of Cytiva.
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