Cyanine5 alkyne

Alkynes Cyanine5 Click chemistry
Cat. # Quantity Price Lead time
A30B0 1 mg $125 in stock
B30B0 5 mg $260 in stock
C30B0 10 mg $325 in stock
D30B0 25 mg $510 in stock
E30B0 50 mg $895 in stock
F30B0 100 mg $1490 in stock
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Cyanine5 is an analog of Cy5®, deeply colored and photostable fluorophore. Cyanine5 alkyne can be attached to various molecules via click chemistry reaction with azides.

This alkyne is non-water soluble, but it can be dissolved in DMF or DMSO before reaction and added to an aqueous reaction mixture. With our labeling protocol, the labeling reaction is very efficient, and high-yielding.

Various substrates bearing azides can be used for the labeling, including azido-labeled biomolecules, polymers, and solid surfaces.

Cyanine5 alkyne, F30B0
Cyanine5 alkyne, F30B0
Cyanine5 alkyne, F30B0
Structure of Cyanine5 alkyne
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Absorbance and emission spectra of Cyanine5

Absorbance and emission spectra of Cyanine5

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General properties

Appearance: dark blue powder
Molecular weight: 556.18
CAS number: 1223357-57-0
Molecular formula: C35H42ClN3O
Solubility: good in dichloromethane, DMF, DMSO, alcohols, very poorly soluble in water (200 mg/L = 0.4 mM)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Peng, Y.-J.; Nanduri, J.; , Wang, N.; Kumar, G.K.; Bindokas, V.;,Paul, B.D.; Chen, X.; Fox, A.P.; Vignane, T.; Filipovic, M.R.; Prabhakar, N.R. Hypoxia sensing requires H2S-dependent persulfidation of olfactory receptor 78. Science Advances, 2023, 9(27), eadf302. doi: 10.1126/sciadv.adf3026
  2. Drekolia, M.-K.; Karantanou, C.; Wittig, I.; Li, Y.; Fuhrmann, D.C.; Brüne, B.; Katsouda, A.; Hu, J.; Papapetropoulos, A.; Bibli, S.-I. Loss of cardiac mitochondrial complex I persulfidation impairs NAD + homeostasis in aging. Redox Biology, 2023, 69, 103014. doi: 10.1016/j.redox.2023.103014
  3. Sokolova, V.; Ebel, J.-F.; Kollenda, S.; Klein, K.; Kruse, B.; Veltkamp, C.; Lange, C. M.; Westendorf, A. M.; Epple, M. Uptake of Functional Ultrasmall Gold Nanoparticles in 3D Gut Cell Models. Small, 2022, 18(31), 2201167. doi: 10.1002/smll.202201167
  4. Sargiacomo, C.; Stonehouse, S.; Moftakhar, Z.; Sotgia, F.; Lisanti, M.P. MitoTracker Deep Red (MTDR) Is a Metabolic Inhibitor for Targeting Mitochondria and Eradicating Cancer Stem Cells (CSCs), With Anti-Tumor and Anti-Metastatic Activity In Vivo. Frontiers in Oncology, 2021, 11, 678343. doi: 10.3389/fonc.2021.678343
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Cy® is a trademark of GE Healthcare.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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