Strained cycloalkynes, like cyclooctynes, react quickly with azides in a reaction called SPAAC, strain-promoted alkyne-azide cycloaddition. The reaction does not require catalyst, it is very rapid and high yielding. The process is useful for the conjugation of different classes of molecules even in vivo.
There is a balance between reactivity and stability of cycloalkynes. Azodibenzocyclooctynes (DBCO, ADIBO) are cyclooctyne derivatives that are characterized by excellent reaction kinetics and very good stability.
Dibenzocyclooctyne (DBCO, ADIBO) containing the green fluorophore AF 488 for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples. Pure 5-isomer.
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Dibenzocyclooctyne (DBCO, ADIBO) containing the yellow fluorophore AF 555 for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.
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Dibenzocyclooctyne (DBCO, ADIBO) containing the orange fluorophore AF 568 for cooper-free labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.
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Dibenzocyclooctyne (DBCO, ADIBO) derivative of the far red fluorophore AF 647 for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.
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BCN-PEG4-Cyanine3 is an ethylene glycol separated linker for the azido-modified molecules for copper-free click conjugation.
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BDP 558/568 DBCO is a bright and photostable dye with emission in the yellow spectrum range. It contains a cyclooctyne substituting group (i.e. DBCO), which can react with various azides in Click Chemistry reactions.
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BDP 581/591 is a borondipyrromethene dye that can be used not only as a fluorescent dye, but also as a ROS probe. It can be conjugated with azides using copper-free click chemistry.
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This red-fluorescent dye contains a substituting group for copper-free click chemistry.
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BDP FL DBCO is a dye cyclooctyne for copper-free click chemistry reactions with azides. Its absorption and emission wavelengths correspond to those of fluorescein (FAM), but BDP FL is significantly more photostable.
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A derivative of BDP R6G dye with azodibenzocyclooctyne (DBCO or ADIBO) for copper-free click chemistry conjugation with azides.
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This yellow-orange-fluorescent boron dipyrromethene dye contains a DBCO substituting group for participation in click chemistry reactions.
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A dye with a dibenzocyclooctyne substituting group for the ROX channel.
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Cyanine3 dibenzocyclooctyne (DBCO, or ADIBO) is a cycloalkyne dye derivative for copper-free click chemistry.
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Cyclooctyne derivative of Cyanine3.5 dye, which easily reacts with various azides resulting in stable conjugates.
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Dibenzocyclooctyne (DBCO, ADIBO) containing Cyanine3B fluorophore for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.
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Cyanine5 dye with dibenzocyclooctyne (DBCO) functional group for copper-free click chemistry.
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Cyanine5.5 is a far-red / NIR emitting dye that is useful for bioimaging studies. Cyanine5.5 DBCO is a derivative of the dye with a cycloalkyne moiety for copper-free click chemistry.
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NIR fluorescent dye functionalized with cyclooctyne moiety for copper free click chemistry conjugation with azides.
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Bifunctional linker containing dibenzocyclooctyne (DBCO, ADIBO) for strain-promoted copper-free click reaction (SPAAC) and free amine for the coupling with various electrophilic compounds like activated esters and epoxides.
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Bifunctional linker containing a thiol-reactive maleimide group and an azide-reactive dibenzocyclooctyne moiety.
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Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain-promoted copper-free click chemistry (SPAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.
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A heterobifunctional linker with DBCO and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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Activated ester for modification of proteins/antibodies/peptides followed by introduction into copper-free click chemistry reaction.
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BCN-containing phosphoramidite for the synthesis of oligonucleotides and their labeling using copper-free click reactions.
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BCN-containing phosphoramidite for the synthesis of oligonucleotides and their labeling using copper-free click reactions.
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BCN-PNP carbonate is a tool to introduce BCN moety via carbamate linkage with primary amino groups of substrate.
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Cyclooctyne derivative of fluorescein (FAM) for copper-free click chemistry reaction with azides. Pure 6-isomer of fluorescein.
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ROX (rhodamine X) is a bright rhodamine dye. This is a DBCO (dibenzocyclooctyne) derivative that has high reactivity against azides.
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sulfo-Cyanine3 DBCO is a cycloalkyne derivative of water-soluble fluorophore sulfo-Cyanine3 for copper-free click chemistry.
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Water-soluble cycloalkyne for copper-free click chemistry reaction with azides. A derivative of sulfo-Cyanine5.
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Sulfo-Cyanine5.5 DBCO is a far-red, water-soluble reagent for copper-free click reaction with azides.
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Dibenzocyclooctyne (DBCO, or ADIBO) conjugated with sulfo-Cyanine7, a water-soluble near-IR dye for labeling azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.
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Cyclooctyne derivative of tetramethylrhodamine (TMR, TAMRA) for copper-free click chemistry conjugation with azides. Pure 5-isomer.
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