AF 647 DBCO

Cycloalkynes AF 647 Copper-free click chemistry

Cat. #	Quantity	Price	Lead time
1G8F0	1 mg	$140	5 days
2G8F0	5 mg	$450	in stock
4G8F0	25 mg	$1460	in stock
5G8F0	50 mg	$1890	in stock
6G8F0	100 mg	$2990	in stock

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Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.

AF 647 is a bright, far-red-emitting fluorescent dye with high fluorescence quantum yield and photostability. AF 647 is a water-soluble, pH-insensitive dye. The spectrum of AF 647 is far from the green-yellow wavelengths, which makes this fluorophore indispensable for the microscopy of tissues with high autofluorescence.

AF 647 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.

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Absorption and emission spectra of AF 647

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General properties

Appearance:	deep-blue powder
Molecular weight:	1266.74
Molecular formula:	C₅₆H₆₈K₃N₄O₁₄S₄
Solubility:	water, DMSO, DMF, methanol
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	655
ε, L⋅mol⁻¹⋅cm⁻¹:	191800
Emission maximum, nm:	680
Fluorescence quantum yield:	0.15
CF₂₆₀:	0.09
CF₂₈₀:	0.08

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.