AF 647 DBCO

Cat. # Quantity Price Lead time
1G8F0 1 mg $140 in stock
2G8F0 5 mg $450 in stock
4G8F0 25 mg $1460 in stock
5G8F0 50 mg $1890 in stock
6G8F0 100 mg $2990 in stock
Found better price? Let us know and we will propose the way forward!

Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.

AF 647 is a bright, far-red-emitting fluorescent dye with high fluorescence quantum yield and photostability. AF 647 is a water-soluble, pH-insensitive dye. The spectrum of AF 647 is far from the green-yellow wavelengths, which makes this fluorophore indispensable for the microscopy of tissues with high autofluorescence.

AF 647 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.

Absorption and emission spectra of AF 647

Absorption and emission spectra of AF 647

Customers also purchased with this product

PreciseGreen dsDNA Quantification Reagent, 200×

PreciseGreen is a dye for precise fluorescence-based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.

AF 568 DBCO

Dibenzocyclooctyne (DBCO, ADIBO) containing the orange fluorophore AF 568 for cooper-free labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.

AF 488 NHS ester

AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.

General properties

Appearance: deep-blue powder
Molecular weight: 1266.74
Molecular formula: C56H68K3N4O14S4
Solubility: water, DMSO, DMF, methanol
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 655
ε, L⋅mol−1⋅cm−1: 191800
Emission maximum, nm: 680
Fluorescence quantum yield: 0.15
CF260: 0.09
CF280: 0.08
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.