Cyanine5.5 phosphoramidite
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2680-100mg | 100 mg | – | in stock | |
| 2680-250mg | 250 mg | $290 | in stock | |
| 2680-1g | 1 g |
$950
|
in stock | |
| 2680-10g | 10 g | please inquire | 5 days |
Cyanine5.5 is a fluorophore with the emission in far-red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.
This phosphoramidite can be used for the synthesis of 5'-labeled oligonucleotides by direct labeling in oligonucleotide synthesizer.
The structure of phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.
Absorption and emission spectra of Cyanine5.5
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FAM phosphoramidite, 5-isomer
Single isomer 5-FAM phosphoramidite for the synthesis of modified oligonucleotides.AF 568 azide
Azide derivative of water-soluble AF 568 dye for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.General properties
| Appearance: | dark colored solid |
| Molecular weight: | 916.61 |
| Molecular formula: | C55H71N5ClO3P |
| Quality control: | NMR 1H, 31P, HPLC-MS (85%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 694 |
| ε, L⋅mol−1⋅cm−1: | 198000 |
| Emission maximum, nm: | 710 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.07 |
| CF280: | 0.03 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | 6 min coupling time recommended |
| Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |
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