FAM NHS ester, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15120 | 1 mg | – | in stock | |
| 25120 | 5 mg | $110 | in stock | |
| 45120 | 25 mg | $145 | in stock | |
| 55120 | 50 mg | $260 | in stock | |
| 65120 | 100 mg |
$470
|
in stock | |
| 85120 | 1 g | please inquire | in stock | |
| 95120 | 5 g | please inquire | in stock |
Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.
Fluorescein is compatible with a wide variety of fluorescent instrumentation - most instruments are equipped with FAM channel filters.
This product contains the pure 6-isomer of fluorescein.
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Cyanine5 maleimide
Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.DMSO (dimethyl sulfoxide), labeling grade
Pure dimethyl sulfoxide (DMSO) solvent with quality optimized for bioconjugation reactions.FAM isothiocyanate (FITC), 5- and 6-isomers
An isothiocyanate derivative of fluorescein for labeling of antibodies and other biomolecules. Mix of 5- and 6-isomers.General properties
| Appearance: | yellow crystalline solid |
| Mass spec M+ increment: | 358.0 |
| Molecular weight: | 473.39 |
| CAS number: | 92557-81-8 |
| Molecular formula: | C25NO9H15 |
| IUPAC name: | (2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| ε, L⋅mol−1⋅cm−1: | 74000 |
| Emission maximum, nm: | 517 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.22 |
| CF280: | 0.17 |
Product citations
- Haynes, J.R.; Whitmore, C.A.; Behof, W.J.; Landman, C.A.; Ong, H.H.; Feld, A.P.; Suero, I.C.; Greer, C.B.; Gore, J.C.; Wijesinghe, P.; Matsubara, J.A.; Wadzinski, B.E.; Spiller, B.W.; Pham, W. Targeting Soluble Amyloid-Beta Oligomers With a Novel Nanobody. Research Square, 2024, preprint. doi: 10.21203/rs.3.rs-3944211/v1
- Chen, Z.; Zakrzewska, S.; Hajare, H.S.; Du Bois, J.; Minor, D.L. Expression, purification, and characterization of anuran saxiphilins using thermofluor, fluorescence polarization, and isothermal titration calorimetry. STAR Protocols, 2024, 5(1), 102792. doi: 10.1016/j.xpro.2023.102792
- Kropacheva, N. O.; Golyshkin, A. A.; Vorobyeva, M. A.; Meschaninova, M. I. Convenient Solid-Phase Attachment of Small-Molecule Ligands to Oligonucleotides via a Biodegradable Acid-Labile P-N-Bond. Molecules, 2023, 28(4), 1904. doi: 10.3390/molecules28041904
- Liu, X.; Ren, X.; Zhou, L.; Liu, K.; Deng, L.; Qing, Q.; Li, J.; Zhi, F.; Li, M. Tollip Orchestrates Macrophage Polarization to Alleviate Intestinal Mucosal Inflammation. Journal of Crohn’s and Colitis, 2022, 16(7), 1151–1167. doi: 10.1093/ecco-jcc/jjac019
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