Cyanine5 phosphoramidite

Cyanine5 Phosphoramidites

Cat. #	Quantity	Price	Lead time
1740-100mg	100 mg	–	in stock
1740-250mg	250 mg	$290	5 days
1740-1g	1 g	$950	in stock
1740-10g	10 g	$6750	21 days

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Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.

This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5′-end, the most typical location of the fluorophore. This is a terminating, non-nucleoside reagent.

Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement - a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases the stability of the reagent, maintaining efficient coupling over a longer time.

Structure of Cyanine5 phosphoramidite, 5'-terminal

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Absorption and emission spectra of Cyanine5

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FAM phosphoramidite, 6-isomer

FAM phosphoramidite 6-isomer, is a widely used reagent in nucleic acid chemistry for labeling oligonucleotides. It is derived from the single pure isomer of 6-carboxyfluorescein and separated from amidite linkage via C6 aminohexanol spacer.

General properties

Appearance:	dark colored solid
Molecular weight:	816.49
Molecular formula:	C₄₇H₆₇N₅ClO₃P
Quality control:	NMR ¹H, ³¹P, HPLC-MS (80%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	646
ε, L⋅mol⁻¹⋅cm⁻¹:	250000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.2
CF₂₆₀:	0.03
CF₂₈₀:	0.04

Oligo synthesis details

Diluent:	acetonitrile
Coupling conditions:	6 min coupling time recommended
Deprotection conditions:	recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.