Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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Bifunctional crosslinker with an amine-reactive succinimidyl ester and a thiol-reactive maleimide group.
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AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.
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NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.
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AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomer
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Bright, water-soluble succinimide ester of AF 647 dye for protein and antibody labeling.
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
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NHS-ester of AMCA dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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N-Succinimidyl 4-Azidotetrafluorobenzoate (ATFB SE, ATFB-OSu) is a photo-activatable linker used as a versatile labeling agent for investigating biological receptors.
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Water-soluble ATT 550 succinimidyl ester for labeling various amine-containing molecules in the aqueous phase.
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A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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Activated NHS ester for azido-labeling of peptides and proteins.
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Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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BDP 576/589 NHS ester is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays. This is an amine-reactive NHS ester derivative.
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Amine-reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel possessing a high molar extinction coefficient and a good emission quantum yield.
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Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays. This compound contains an aminohexanoic acid (C6) linker between the dye and the functional group.
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BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.
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BDP TR-X-NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used to conjugate proteins and peptides.
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PEG4-containing activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules.
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Biotin derivative with C6-spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines.
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Biotin derivative with long spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines.
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Cyanine2 NHS ester minimal dye for protein labeling, an equivalent of Cy2 NHS ester minimal dye.
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Amine-reactive Cyanine3 dye NHS ester for the labeling of amino-groups.
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Cyanine3 minimal dye for protein labeling, and analog of Cy3 minimal dye.
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Cyanine3.5 dye NHS ester derivative the labeling of amino-groups in biomolecules.
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Cyanine3B NHS ester is a reactive dye with significantly increased fluorescence quantum yield and photostability for the specific labeling of amino-groups in biomolecules.
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Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.
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Cy5 NHS ester minimal dye for protein labeling, an analog of Cy5 minimal dye.
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Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.
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Amine reactive NHS ester of near infrared fluorescent dye, an improved analog of Cy7®.
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Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5 .
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Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain-promoted copper-free click chemistry (SPAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.
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A heterobifunctional linker with DBCO and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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Homobifunctional crosslinker with two NHS ester groups at each end of a short 5-atom (7.7 angstrom) spacer.
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Bifunctional linker with DOTA chelating and amine-reactive NHS ester groups for bioconjugation.
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Bifunctional linker with DOTA chelating and amine-reactive NHS ester groups for bioconjugation.
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Functionalized DusQ 1 quencher derivative with quenching range from 480 - 580 nm for labeling of amine-containing molecules.
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DusQ 2 dark quencher with a quenching range from 560 - 670 nm with primary amine reactivity for synthesis of FRET probes. This NHS ester can be coupled to various molecules bearing amino groups.
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Amino-reactive NHS ester with quencher for the conjugation with amines-containing molecules, DusQ 21 quenching range 590 - 720 nm.
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Amino-reactive NHS ester for the labeling with far-red quencher DusQ 3.
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Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.
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NHS-ester of HEX dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups.
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Hexynoic acid NHS ester for the labeling of biomolecules with alkyne group for copper-catalyzed click chemistry.
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JOE is a fluorescent dye with emission in the yellow part of the spectrum. This derivative is an activated NHS ester for conjugation with amines.
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Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.
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R6G (rhodamine 6G) NHS ester, pure 5-isomer. This dye is often used for the labeling of oligonucleotides for qPCR.
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Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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Pure 6-isomer of ROX (Rhodamine X, R101) NHS-ester for amine labeling.
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Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.
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Amine-reactive activated ester of water-soluble, sulfonated sulfo-Cyanine3.5 dye.
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Bifunctional derivative of sulfo-Cyanine5 dye containing reactive groups of terminal alkyne and NHS ester.
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Water soluble amine reactive Cyanine5 dye with two NHS ester groups.
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Water-soluble sulfo-Cyanine5 succinimidyl ester for labeling various amine-containing molecules in the aqueous phase.
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Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.
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sulfo-Cyanine7 bis-NHS ester is a bifunctional derivative of water-soluble near-infrared sulfo-Cyanine7. It contains two activated ester functional groups.
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sulfo-Cyanine7 NHS ester is a water-soluble, near-infrared, amine-reactive dye.
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A water-soluble near-infrared emitting dye for in vivo imaging applications. sulfo-Cyanine7.5 is an analog of indocyanine green (ICG) possessing higher emission quantum yield and a linker arm.
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Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides. Pure 5-isomer.
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NHS-ester of TAMRA dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups. Pure 6-isomer.
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A heterobifunctional linker with trans-cyclooctene and NHS ester groups flanking PEG4 (tetraethylene glycol) for labeling of antibodies, proteins, and other primary amine-containing biomolecules.
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Thiazole Orange NHS ester for labeling biomolecules (peptides, proteins, amino-DNA, etc.). Thiazole orange is a cyanine dye that is essentially dark in solution but has a bright fluorescence in double-stranded DNA and RNA.
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TR is a red-emitting fluorescent dye based on sulforhodamine scaffold, an amine-reactive NHS ester.
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