AMCA NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3606-5mg | 5 mg | $110 | in stock | |
| 3606-25mg | 25 mg | $290 | in stock | |
| 3606-50mg | 50 mg |
$435
|
in stock | |
| 3606-100mg | 100 mg |
$595
|
in stock |
Activated NHS-ester of AMCA (aminomethyl coumarin acetate) dye. This NHS ester is an amine-reactive dye for labeling of amine groups in proteins, peptides, amino-modified oligos, and other target molecules.
AMCA is one of the brightest blue fluorescent dyes. This fluorophore has a relatively large Stoke’s shift, high resistance to photobleaching, and pH-independent fluorescence from pH 4 to 10. AMCA is a widely used fluorophore for multiple-color labeling due to its minimal fluorescence overlap with green- and longer wavelength-emitting fluorescent dyes.
Absorption and emission spectra of AMCA
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AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.General properties
| Appearance: | gray powder |
| Mass spec M+ increment: | 331.3 |
| Molecular weight: | 366.76 |
| CAS number: | 113721-87-2 |
| Molecular formula: | C16H15ClN2O6 |
| Solubility: | in DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 348 |
| ε, L⋅mol−1⋅cm−1: | 17400 |
| Emission maximum, nm: | 435 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.13 |
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