JOE NHS ester, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11120 | 1 mg | $110 | in stock | |
| 21120 | 5 mg | $210 | in stock | |
| 41120 | 25 mg |
$410
|
in stock | |
| 51120 | 50 mg |
$695
|
in stock | |
| 61120 | 100 mg |
$1190
|
in stock |
JOE is a fluorescein-derived xanthene dye containing methoxy groups and chlorine atoms. Its absorption and emission spectra are between FAM and TAMRA/ROX channels. For this reason, this fluorophore is often used for multiplex detection, including DNA sequencing.
This derivative is an activated N-hydroxysuccinimide ester used to label proteins, peptides, amino-modified oligonucleotides, and other target molecules via conjugation with amines.
Absorption and emission spectra of JOE dye
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Amine-reactive activated ester of water-soluble, sulfonated sulfo-Cyanine3.5 dye.FAM NHS ester, 6-isomer
Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.General properties
| Appearance: | orange powder |
| Molecular weight: | 602.33 |
| CAS number: | 113394-24-4 |
| Molecular formula: | C27H17Cl2NO11 |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| Fluorescence quantum yield: | 0.61 |
| CF260: | 0.36 |
| CF280: | 0.28 |
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