sulfo-Cyanine5 antibody labeling kit
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3321-1rxn |
1 rxn
|
$69 | 1 days | |
| 3321-10rxn |
10 rxn
|
$397 | 1 days |
A ready-to-use kit for labeling antibodies with sulfo-Cyanine5 dye. This kit contains all necessary components (chemicals and consumables) to perform 10 labelings of antibodies (up to 100 ug antibody per each reaction). Reaction clean-up is achieved using enclosed gel filtration spin columns.
Precise quantification of the dye guarantees the reproducible outcome of the labeling and number of fluorophores per antibody molecule.
Purified labeled antibodies are available in PBS buffer after 40 min (10 min hands-on time). The kit tolerates common additives such as Tris, glycerol, and sodium azide.
Product in action
Human blood smear stained with green fluorescent nuclear stain LUCS13 and antibodies to neutrophil myeloperoxidase
Human blood smear stained with blue fluorescent nuclear stain DAPI and antibodies to neutrophil myeloperoxidase
Absorption and emission spectra of sulfo-Cyanine5
Ready-to-use kit manual
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General properties
| Storage conditions: | Store at temperature between +4 °C and +20 °C. Do not freeze! |
| MSDS: | Download |
Kit
| Storage time in months: | 12 |
References
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- Rana, H.; Truong, N.R.; Johnson, B.; Baharlou, H.; Herbert, J.J.; Kandasamy, S.; Goddard, R.; Cohen, R.C.; Wines, M.; Nasr, N.; Harman, A.N.; Bertram, K.M.; Sandgren, K.J.; Cunningham, A.L. Herpes simplex virus spreads rapidly in human foreskin, partly driven by chemokine-induced redistribution of Nectin-1 on keratinocytes. PLOS Pathogens, 2024, 20(6), e1012267. doi: 10.1371/journal.ppat.1012267
- Kirikovich, S.S.; Levites, E.V.; Proskurina, A.S.; Ritter, G.S.; Peltek, S.E.; Vasilieva, A.R.; Ruzanova, V.S.; Dolgova, E.V.; Oshihmina, S.G.; Sysoev, A.V.; Koleno, D.I.; Danilenko, E.D.; Taranov, O.S.; Ostanin, A.A.; Chernykh, E.R.; Kolchanov, N.A.; Bogachev, S.S. The Molecular Aspects of Functional Activity of Macrophage-Activating Factor GcMAF. International Journal of Molecular Sciences, 2023, 24(24), 17396. doi: 10.3390/ijms242417396
- Rhodes, J. W.; Botting, R. A.; Bertram, K. M.; Vine, E. E.; Rana, H.; Baharlou, H.; Vegh, P.; O’Neil, T. R.; Ashhurst, A. S.; Fletcher, J.; Parnell, G. P.; Graham, J. D.; Nasr, N.; Lim, J. J. K.; Barnouti, L.; Haertsch, P.; Gosselink, M. P.; Di Re, A.; Reza, F.; Ctercteko, G.; Jenkins, G. J.; Brooks, A. J.; Patrick, E.; Byrne, S. N.; Hunter, E.; Haniffa, M. A.; Cunningham, A. L.; Harman, A. N. Human Anogenital Monocyte-Derived Dendritic Cells and Langerin+cDC2 Are Major HIV Target Cells. Nat Commun, 2021, 12(1), 2147. doi: 10.1038/s41467-021-22375-x
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