Cyanine5.5 NHS ester

Cat. # Quantity Price Lead time
17020 1 mg
$139.90
in stock
27020 5 mg
$259.90
in stock
47020 25 mg
$489.90
in stock
57020 50 mg
$939.90
in stock
67020 100 mg
$1799.00
in stock
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Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5® and DyLight 680.

Cy5.5 absorbance and emission spectra

Cy5.5 absorbance and emission spectra

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General properties

Appearance: dark blue to violet solid
Molecular weight: 767.66
CAS number: 2375105-86-3
Molecular formula: C44H46N3BF4O4
IUPAC name: 1H-​Benz[e]​indolium, 2-​[5-​(1,​3-​dihydro-​1,​1,​3-​trimethyl-​2H-​benz[e]​indol-​2-​ylidene)​-​1,​3-​pentadien-​1-​yl]​-​3-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​1,​1-​dimethyl-
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 684
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Product citations

  1. Guo, H.; Hu, Q.; Yu, X.; Shen, L.; Dai, T.; Yang, S.; Dou, H. Functional Ligand-Mediated Tailoring of Size and Spatial Architecture in Metal-Organic Framework/Polymer Hybrids for Photothermal Applications. Journal of Colloid and Interface Science, 2026, 702, 138963. doi: 10.1016/j.jcis.2025.138963
  2. Song, B.; Na, Y.-G.; Kim, B. J.; Jin, M.; Song, Y. H.; Kim, D.-E.; Hwang, S.; Baek, J.-S.; Lee, H.-K.; Cho, C.-W. Platelet Membrane-Coated Poly (Lactic-Co-Glycolic Acid) Nanoparticles as a Targeting Drug Delivery System for Multidrug-Resistant Breast Cancer. IJN, 2025, 20, 8529–8545. doi: 10.2147/IJN.S517753
  3. Jang, K.; Kajale, S. N.; Joy, B. C.; Bono, D. C.; Neltner, B.; Sarkar, D. A Wearable Device for Continuous Monitoring of Circulating Cells at Single-Cell Resolution. npj Biosensing, 2025, 2(1), 10. doi: 10.1038/s44328-025-00032-3
  4. Muradova, Z.; Carmès, L.; Brown, N.; Rossetti, F.; Guthier, R.; Yasmin-Karim, S.; Lavelle, M.; Morris, T.; Guidelli, E. J.; Isikawa, M.; Dufort, S.; Bort, G.; Tillement, O.; Lux, F.; Berbeco, R. Targeted-Theranostic Nanoparticles Induce Anti-Tumor Immune Response in Lung Cancer. Journal of Nanobiotechnology, 2025, 23(1), 466. doi: 10.1186/s12951-025-03542-4
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