Cyanine3 tetrazine
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 110E0 | 1 mg | $125 | in stock | |
| 210E0 | 5 mg | $260 | in stock | |
| 410E0 | 25 mg |
$510
|
in stock | |
| 510E0 | 50 mg |
$895
|
in stock | |
| 610E0 | 100 mg |
$1490
|
in stock |
Cyanine3 tetrazine is a fluorescent dye with tetrazine moiety for a conjugation reaction called TCO (trans-cyclooctene) ligation. Actually, tetrazines react not only with trans-cyclooctenes, but also with cyclopropenes and strained non-benzo-fused cycloalkynes.
Cyanine3 is a moderately hydrophobic dye, enabling labeling in non-aqueous environment as well as in aqueous media containing some organic co-solvent like acetonitrile or DMSO. For reactions in purely aqueous media, a sulfonated version of this reagent is available.
Absorption and emission spectra of Cyanine3
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FAM tetrazine, 6-isomer
This molecule is a conjugate of bright fluorophore FAM (fluorescein) and tetrazine, a reactive group that reacts with trans-cycloalkenes in a reaction called IEDDA (inverse electron demand Diels-Alder reaction).sulfo-Cyanine7 tetrazine
Near infrared, water soluble dye methyltetrazine for the conjugation with strained cycloalkenes in TCO ligation reaction.General properties
| Appearance: | dark red solid |
| Mass spec M+ increment: | 611.4 |
| Molecular weight: | 727.64 |
| CAS number: | 2183473-57-4 |
| Molecular formula: | C40H46N7BF4O |
| Solubility: | good in DCM, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Bai, M.; Cao, X.; Huang, W.; Chen, F.; Zhao, Y.; Xue, J.; Li, Y.; Cheng, Y.; Zhang, L.; Zhao, Y. Visualizing Newly Synthesized RNA by Bioorthogonal Labeling-primed DNA Amplification. Analytical Chemistry, 2020, 92(12), 8444–8449. doi: 10.1021/acs.analchem.0c01161
- Moutsiopoulou, A.; Broyles, D.; Joda, H.; Dikici, E.; Kaur, A.; Kaifer, A.E.; Daunert, S.; Deo, S.K. Bioluminescent Protein-Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System. Analytical Chemistry, 2020, 92(11), 7393–7398. doi: 10.1021/acs.analchem.0c00518
- Chen, F.; Bai, M.; Cao, X.; Zhao, Y.; Xue, J.; Zhao, Y. Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures. Nucleic Acids Research, 2019, 47(22), e145. doi: 10.1093/nar/gkz852
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