AF 594 tetrazine, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 178E0 | 1 mg | $198 | in stock | |
| 278E0 | 5 mg |
$736
|
in stock | |
| 478E0 | 25 mg |
$2396
|
in stock |
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06/16/2025
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AF 594 is a water-soluble red-fluorescent dye with high fluorescence quantum yield and high photostability. The dye is similar to Texas Red in spectral characteristics (absorption max. at 586 nm, emission max. at 613 nm) and is not sensitive to pH changes within the range from 4 to 10. This reagent is a pure 6-isomer.
This AF 594 derivative contains tetrazine moiety that reacts with trans-cycloalkenes and other strained olefins in inverse electron demand Diels-Alder reaction (IEDDA). The reaction is very quick, specific and suitable for in vitro cell labeling.
Absorption and emission spectra of AF 594
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Fluorescent dye for detection of amplification in real-time PCR in a ready-to-use 100× stock solution for adding to the master mix.General properties
| Appearance: | dark violet crystals |
| Molecular weight: | 906.01 |
| Molecular formula: | C45H43N7O10S2 |
| Solubility: | good in methanol, DMSO, DMF, and water |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 586 |
| ε, L⋅mol−1⋅cm−1: | 105000 |
| Emission maximum, nm: | 613 |
| Fluorescence quantum yield: | 0.77 |
| CF260: | 0.28 |
| CF280: | 0.51 |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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