AF 594 tetrazine, 6-isomer

Cat. # Quantity Price Lead time
178E0 1 mg $198 in stock
278E0 5 mg $736 in stock
478E0 25 mg $2396 in stock
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AF 594 is a water-soluble red-fluorescent dye with high fluorescence quantum yield and high photostability. The dye is similar to Texas Red in spectral characteristics (absorption max. at 586 nm, emission max. at 613 nm) and is not sensitive to pH changes within the range from 4 to 10. This reagent is a pure 6-isomer.

This AF 594 derivative contains tetrazine moiety that reacts with trans-cycloalkenes and other strained olefins in inverse electron demand Diels-Alder reaction (IEDDA). The reaction is very quick, specific and suitable for in vitro cell labeling.

Absorption and emission spectra of AF 594

Absorption and emission spectra of AF 594

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General properties

Appearance: dark violet crystals
Molecular weight: 906.01
Molecular formula: C45H43N7O10S2
Solubility: good in methanol, DMSO, DMF, and water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 586
ε, L⋅mol−1⋅cm−1: 105000
Emission maximum, nm: 613
Fluorescence quantum yield: 0.77
CF260: 0.28
CF280: 0.51
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