AF 594 tetrazine, 6-isomer

Tetrazines AF 594 Copper-free click chemistry

Cat. #	Quantity	Price	Lead time
178E0	1 mg	$198	in stock
278E0	5 mg	$736	in stock
478E0	25 mg	$2396	in stock

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AF 594 is a water-soluble red-fluorescent dye with high fluorescence quantum yield and high photostability. The dye is similar to Texas Red in spectral characteristics (absorption max. at 586 nm, emission max. at 613 nm) and is not sensitive to pH changes within the range from 4 to 10. This reagent is a pure 6-isomer.

This AF 594 derivative contains tetrazine moiety that reacts with trans-cycloalkenes and other strained olefins in inverse electron demand Diels-Alder reaction (IEDDA). The reaction is very quick, specific and suitable for in vitro cell labeling.

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Absorption and emission spectra of AF 594

General properties

Appearance:	dark violet crystals
Molecular weight:	906.01
Molecular formula:	C₄₅H₄₃N₇O₁₀S₂
Solubility:	good in methanol, DMSO, DMF, and water
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	586
ε, L⋅mol⁻¹⋅cm⁻¹:	105000
Emission maximum, nm:	613
Fluorescence quantum yield:	0.77
CF₂₆₀:	0.28
CF₂₈₀:	0.51

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.