(5,6)-FAM diacetate NHS ester
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
2934-1mg | 1 mg | – | in stock | |
2934-25mg | 25 mg | $160 | in stock | |
2934-50mg | 50 mg | $270 | in stock | |
2934-100mg | 100 mg | $520 | in stock |
(5,6)-FAM diacetate NHS ester is a cell-permeable amine-reactive label for biomolecules.
This molecule is non-fluorescent until the acetate groups are cleaved by intracellular esterases resulting in the formation of a highly fluorescent fluorophore with emission in the green spectrum range (absorption maximum at ~492 nm, emission maximum at ~517 nm). Due to this characteristic, this product is used for tracking cells and flow cytometric evaluations and studying intracellular esterase.
(5,6)-FAM diacetate absorbance and emission spectra
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A reagent for detecting reactive oxygen species in living cells.AF 343 (Coumarin)-X-azide
AF 343 (Coumarin) is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by сlick chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility and provides spatial separation between the dye and the biomolecule.General properties
Appearance: | white solid |
Molecular weight: | 557.46 |
CAS number: | 150347-59-4 |
Molecular formula: | C29H19NO11 |
Solubility: | good in DMF, DMSO |
Quality control: | NMR 1H and 31P, HPLC-MS (95%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 492 |
Emission maximum, nm: | 517 |
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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