DiD, lipophilic tracer

Neuroscience Microscopy Flow cytometry Cell membrane

Cat. #	Quantity	Price	Lead time
3251-1mg	1 mg	–	in stock
3251-5mg	5 mg	$65.00	in stock (MOQ 2 pcs.)
3251-50mg	50 mg	$238.00	in stock

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DiD is a far-red fluorescent carbocyanine dye for cell membrane labeling in vivo and in vitro. DiD diffuses laterally to stain the entire cell, allowing it to be used as an anterograde and retrograde tracer of neurons. In intact tissue, the dye does not transfer from labeled to unlabeled cells, but some transfer may occur when the membrane is disrupted, for example, after sectioning. The dye is weakly fluorescent until incorporated into membranes.

DiD can be used with other tracers in dual-color studies, such as DiI or DiO.

DiD is a solid form for the direct application of crystals to membranes.

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Human peripheral blood neutrophils stained with DiD (3251-x) lipophilic tracer and DAPI (2996-x) nuclear stain

Absorption and emission spectra of DiD

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Can I use Lumiprobe's dyes for two-photon microscopy? What excitation and emission wavelengths do they have?

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General properties

Appearance:	dark blue solid
Molecular weight:	987.38
CAS number:	75539-51-4
Molecular formula:	C₆₁H₉₉IN₂
Solubility:	DMSO
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	647
Emission maximum, nm:	666

Product citations

Dalinina, V. D.; Shashkovskaya, V. S.; Khaskhanova, I. M.; Travnikova, D. Y.; Chmelyuk, N. S.; Korzhenevskiy, D. A.; Belousov, V. V.; Abakumova, T. O. Effect of Acoustic Pressure on Temozolomide-Loaded Oleic Acid-Based Liposomes and Its Safety to Brain Tissue. Pharmaceuticals, 2025, 18(6), 910. doi: 10.3390/ph18060910
Nazarenko, A. S.; Biryukova, Y. K.; Trachuk, K. N.; Orlova, E. A.; Vorovitch, M. F.; Pestov, N. B.; Barlev, N. A.; Levaniuk, A. I.; Gordeychuk, I. V.; Lunin, A. S.; Demyashkin, G. A.; Shegai, P. V.; Kaprin, A. D.; Ishmukhametov, A. A.; Kolyasnikova, N. M. Immunotherapeutic Potential of the Yellow Fever Virus Vaccine Strain 17D for Intratumoral Therapy in a Murine Model of Pancreatic Cancer. Vaccines, 2025, 13(1), 40. doi: 10.3390/vaccines13010040
Sindeeva, O.A.; Demina, P.A.; Kozyreva, Z.V.; Terentyeva, D.A.; Gusliakova, O.I.; Muslimov, A.R.; Sukhorukov, G.B. Single Mesenchymal Stromal Cell Migration Tracking into Glioblastoma Using Photoconvertible Vesicles. Nanomaterials, 2024, 14(14), 1215. doi: 10.3390/nano14141215
Liu, X.; Cao, Z.; Liu, N.; Gao, G.; Du, M.; Wang, Y.; Cheng, B.; Zhu, M.; Jia, B.; Pan, L.; Zhang, W.; Jiang, Y.; He, W.; Xu, L.; Zhang, W.; An, Q.; Guo, Q.; Gu, J. Kill two birds with one stone: Engineered exosome-mediated delivery of cholesterol modified YY1-siRNA enhances chemoradiotherapy sensitivity of glioblastoma. Frontiers in Pharmacology, 2022, 13, 975291. doi: 10.3389/fphar.2022.975291

Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.