EdU (5-Ethynyl-2'-deoxyuridine)

Cell proliferation Alkynes Click chemistry
Cat. # Quantity Price Lead time
A0540 5 mg –   in stock
10540 100 mg $120 in stock
20540 250 mg $195 in stock
30540 500 mg $345 in stock
40540 1 g $590 in stock
60540 5 g please inquire in stock
Bulk inquiry
Found better price? Let us know and we will propose the way forward!

EdU (5-ethynyl-2’-deoxyuridine) is a nucleoside, an analog of thymidine, carrying an ethynyl group. This nucleoside behaves as a substrate for cellular DNA replication machinery. After EdU incorporation, DNA containing ethynyl groups can be ligated by click chemistry reaction with various dye azides. Fluorescent DNA can be detected with microscopy, or cells can be sorted with FACS.

Dividing human melanoma cells A375. 5-ethynyl-2'-deoxyuridine (EdU, x0540, 10 μM) was added to the cell culture 2 h prior to fixation and CuAAC staining with AF488 azide (x1830) and DAPI nuclear stain.
EdU (5-ethynyl-2'-deoxyuridine)
EdU (5-ethynyl-2'-deoxyuridine)
EdU (5-ethynyl-2'-deoxyuridine)
EdU (ethynyl deoxyuridine) structure
Download in
ChemDraw format

Recommended protocol

Calculator

Customers also purchased with this product

Biotin-11-UTP

Biotinylated UTP for enzymatic RNA labeling.

FAM maleimide, 6-isomer

Fluorescein (FAM) maleimide is a thiol reactive dye derived from isomerically pure 6-FAM. Fluorescein is a bright dye that is compatible with various fluorescence measurement instruments.

BDP® 630/650 azide

BDP 630/650 is a bright borondipyrromethene-based fluorophore for the far red area of the spectrum. It possesses a high extinction coefficient and quantum yield. This is an azide for сlick chemistry labeling.

General properties

Appearance: light beige to light brown solid
Molecular weight: 252.22
CAS number: 61135-33-9
Molecular formula: C11H12N2O5
Solubility: in water, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
MSDS: Download
Product specifications

Product citations

  1. Kim, R.; Allbritton, N.L. A Microphysiological System with an Anaerobic Air‐Liquid Interface and Functional Mucus Layer for Coculture of Intestinal Bacteria and Primary Human Colonic Epithelium. Advanced Materials Interfaces, 2024, 11(25), 2400093. doi: 10.1002/admi.202400093
  2. Goedhart, J. Studentsourcing—Aggregating and reusing data from a practical cell biology course. PLOS Computational Biology, 2024, 20(2), e1011836. doi: 10.1371/journal.pcbi.1011836
  3. Dopeso, H.; Rodrigues, P.; Cartón-García, F.; Macaya, I.; Bilic, J.; Anguita, E.; Jing, L.; Brotons, B.; Vivancos, N.; Beà, L.; Sánchez-Martín, M.; Landolfi, S.; Hernandez-Losa, J.; Ramon y Cajal, S.; Nieto, R.; Vicario, M.; Farre, R.; Schwartz Jr, S.; van Ijzendoorn, S.C.D.; Kobayashi, K.; Martinez-Barriocanal, Á.; Arango, D. RhoA downregulation in the murine intestinal epithelium results in chronic Wnt activation and increased tumorigenesis. iScience, 2024, 27(4), 109400. doi: 10.1016/j.isci.2024.109400
  4. Alruwaili, M.M.; Zonneville, J.; Naranjo, M.N.; Serio, H.; Melendy, T.; Straubinger, R.M.; Gillard, B.; Foster, B.A.; Rajan, P.; Attwood, K.; Chatley, S.; Iyer, R.; Fountzilas, C.; Bakin, A.V. A synergistic two-drug therapy specifically targets a DNA repair dysregulation that occurs in p53-deficient colorectal and pancreatic cancers. Cell Reports Medicine, 2024, 5(3), 101434. doi: 10.1016/j.xcrm.2024.101434
Show more (50)
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.