Copper(II)-TВTA catalytic buffer, 1.5x

Click chemistry

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61150	55 mL	$49	in stock

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Ready-to-use catalytic buffer for click chemistry (pH 7.0) containing copper(II), TBTA, triethylammonium acetate, and DMSO. The buffer has a concentration of 1.5×. This reagent is suitable for modifying nucleic acids such as oligonucleotides and small molecules.

Copper(II) should be reduced to copper(I) immediately before reaction. Ascorbic acid is a recommended reductant.

Recommended protocol

Conjugation of Alkyne-modified Oligonucleotides with Dye Azides

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General properties

Appearance:	bluish liquid
Storage conditions:	Storage: 24 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
MSDS:	Download
	Product specifications

Product citations

Ren, J.; Zhou, H.; Zeng, H.; Wang, C.K.; Huang, J.; Qiu, X.; Sui, X.; Li, Q.; Wu, X.; Lin, Z.; Lo, J.A.; Maher, K.; He, Y.; Tang, X.; Lam, J.; Chen, H.; Li, B.; Fisher, D.E.; Liu, J.; Wang, X. Spatiotemporally resolved transcriptomics reveals the subcellular RNA kinetic landscape. Nature Methods, 2023, 20, 695-705. doi: 10.1038/s41592-023-01829-8

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.