JOE azide, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A1130 | 1 mg | $110 | in stock | |
| B1130 | 5 mg | $210 | in stock | |
| C1130 | 10 mg | $310 | in stock | |
| D1130 | 25 mg |
$410
|
in stock | |
| E1130 | 50 mg |
$695
|
in stock | |
| F1130 | 100 mg |
$1190
|
in stock |
JOE is a fluorescent dye with the emission in yellow region of the spectrum. The fluorophore matches channel of HEX dye, another chlorinated fluorescein.
JOE dye is often used in qPCR; oligo labeling can be achieved using сlick chemistry. This is an azide derivative of JOE for сlick chemistry, pure 5-isomer.
Absorption and emission spectra of JOE dye
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Azide derivative of Cyanine3 fluorescent dye for сlick сhemistry.Cyanine3.5 azide
Cyanine3.5 azide for biomolecule labeling through click chemistry.General properties
| Appearance: | red solid |
| Mass spec M+ increment: | 586.1 |
| Molecular weight: | 587.37 |
| CAS number: | 1422178-11-7 |
| Molecular formula: | C26H20N4Cl2O8 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| Fluorescence quantum yield: | 0.61 |
| CF260: | 0.36 |
| CF280: | 0.28 |
Product citations
- Taskova, M.; Astakhova, K. Fluorescent Oligonucleotides with Bis(prop-2-yn-1-yloxy)butane-1,3-diol Scaffold Rapidly Detect Disease-Associated Nucleic Acids. Bioconjugate Chemistry, 2019, 30(12), 3007–3012. doi: 10.1021/acs.bioconjchem.9b00746
- Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
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