AF 488 DBCO
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
118F0 | 1 mg | $140 | in stock | |
218F0 | 5 mg | $450 | in stock | |
418F0 | 25 mg | $1460 | in stock | |
518F0 | 50 mg | $1890 | in stock | |
618F0 | 100 mg | $2640 | in stock |
Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.
AF 488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.
AF 488 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.
Absorption and emission spectra of AF 488
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AF 568 derivative designed for fluorescence microscopy and flow cytometry. Pure 5-isomer.TCO-PEG4-NHS ester (axial isomer)
A heterobifunctional linker with trans-cyclooctene and NHS ester groups flanking PEG4 (tetraethylene glycol) for labeling of antibodies, proteins, and other primary amine-containing biomolecules.General properties
Appearance: | orange solid |
Molecular weight: | 936.08 |
Molecular formula: | C48H49N5O11S2 |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 495 |
Emission maximum, nm: | 519 |
Fluorescence quantum yield: | 0.91 |
Product citations
- Xia, Q.; Perera, H.A.; Bolarinho, R.; Piskulich, Z.A.; Guo, Z.; Yin, J.; He, H.; Li, M.; Ge, X.; Cui, Q.; Ramström, O.; Yan, M.; Cheng, J.-X. Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe. bioRxiv, 2024, preprint. doi: 10.1101/2024.03.08.584185
- Xu, W.; He, M.; Lu, Q. Fibronectin Connecting Cell Sheet Based on Click Chemistry for Wound Repair. Advanced Science, 2024, 11(11), 2306746. doi: 10.1002/advs.202306746