exo-BCN CE-phosphoramidite

Cycloalkynes Oligonucleotide synthesis Phosphoramidites Copper-free click chemistry

Cat. #	Quantity	Price	Lead time
4396-100mg / 8 mL septum	100 mg	–	21 days
4396-250mg / 8 mL septum	250 mg	$790	7 days
4396-1g / 8 mL septum	1 g	$2340	21 days
4396-5g / 8 mL septum	5 g	please inquire	21 days

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BCN-containing phosphoramidite is added at the 5’-terminus of an oligonucleotide. BCN is reactive both to azides (strain-promoted azyde-alkyne cycloaddition, SPAAC) and tetrazines (inverse electron demand Diels-Alder reaction, IEDDA) .

Coupling time is standard, like for amidites of natural nucleosides. Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.

Use standard conditions for deprotection and ammonia solution, or AMA mixture (ammonium hydroxide / 40% methylamine, 1:1) for 2 hours at room temperature.

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General properties

Appearance:	yellowish oil
Mass spec M+ increment:	343.11
Molecular weight:	481.57
Molecular formula:	C₂₄H₄₀N₃O₅P
Solubility:	good in acetonitrile
Quality control:	NMR ¹H, NMR ³¹P (95 %)
Storage conditions:	12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.

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