exo-BCN CE-phosphoramidite

Cycloalkynes Oligonucleotide synthesis Copper-free click chemistry Phosphoramidites

Cat. #	Quantity	Price	Lead time
4396-100mg / septum vial	100 mg	–	21 days
4396-250mg / septum vial	250 mg	$919.90	in stock
4396-1g / septum vial	1 g	$2699.00	in stock
4396-5g / septum vial	5 g	please inquire	in stock

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BCN-containing phosphoramidite is added at the 5′-terminus of an oligonucleotide. BCN is reactive both to azides (strain-promoted azyde-alkyne cycloaddition, SPAAC) and tetrazines (inverse electron demand Diels-Alder reaction, IEDDA) .

The exo-stereoisomer bicyclononine in exo-BCN CE-phosphoramidite exhibits a cycloaddition rate that does not differ significantly from its endo-conformer, indicating that the choice of isomer is not critical for application.

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General properties

Appearance:	yellowish oil
Mass spec M+ increment:	343.11
Molecular weight:	481.57
Molecular formula:	C₂₄H₄₀N₃O₅P
Solubility:	good in acetonitrile
Quality control:	NMR ¹H, NMR ³¹P (95 %)
Storage conditions:	12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Oligo synthesis details

Diluent:	dry acetonitrile
Coupling conditions:	coupling time 6 minutes. Oxidation: 0.5M (1S)-(+)-(10-camphorsulfonyl)-oxaziridine (CSO) in dry acetonitrile, 2 minutes. The modification is incompatible with iodine oxidation. Exclude the dimethoxytrityl (DMT) removal step (as in DMT-on protocol), because the modification is acid sensitive.
Deprotection conditions:	AMA (40% aq. methylamine, 25% ammonia, 1:1 mixture), 2 hours, room temperature

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.