FAM isothiocyanate (FITC), 5-isomer

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4641-1g 1 g $276 in stock
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FAM-5-isothiocyanate or 5-FITC is an isothiocyanate derivative of fluorescein that reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules. It is often used to label antibodies and other probes for use in various fluorescence-based techniques such as microscopy, flow cytometry, and immunofluorescence assays like immunochemistry, Western blotting, and ELISA.

FAM absorbance and emission spectra

FAM absorbance and emission spectra

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An isothiocyanate derivative of fluorescein for labeling of antibodies and other biomolecules. Mix of 5- and 6-isomers.

FAM maleimide, 6-isomer

Fluorescein (FAM) maleimide is a thiol reactive dye derived from isomerically pure 6-FAM. Fluorescein is a bright dye that is compatible with various fluorescence measurement instruments.

HEX NHS ester, 6-isomer

NHS-ester of HEX dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups.

General properties

Appearance: orange powder
Molecular weight: 389.39
CAS number: 3326-32-7
Molecular formula: C21H11NO5S
Solubility: good in DMF or DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
Emission maximum, nm: 517
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