AF 488 TFP ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 118T0 | 1 mg | $165 | 21 days | |
| 218T0 | 5 mg |
$510
|
in stock | |
| 418T0 | 25 mg |
$1650
|
in stock |
AF 488 is a bright and photostable dye well suited for labeling sensitive proteins and antibodies due to its hydrophilicity. AF 488 is a sulfonated derivative of Rhodamine 110 (R110), and, like other rhodamines, AF 488 is represented by two (5- and 6-) isomers. Both isomers have almost identical photophysical properties but require their separation since using a mixture of isomers leads to the «doubling» of the peaks of the labeled products during HPLC and electrophoretic separation. This product contains isomerically pure 5-AF 488.
Tetrafluorophenyl (TFP) esters belong to a group of activated esters widely used to conjugate fluorophores to the primary and secondary amines of biomolecules. Like hydroxysuccinimide (NHS) esters, they form a strong amide bond between the dye and the compound being modified; however, TFP esters are more stable at the basic pH values typically associated with reactions with amines and are also less susceptible to spontaneous hydrolysis.
Absorption and emission spectra of AF 488
Recommended protocol
Calculator
Customers also purchased with this product
AF 532 NHS ester
NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.BDP® 558/568 maleimide
Maleimide derivative of boron-dipyrromethene dye BDP 558/568, which emits fluorescence in the yellow range; BDP 558/568 maleimide can be used for modification of thiol groups in biomolecules.AF 594 NHS ester
Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomerGeneral properties
| Appearance: | red crystals |
| Molecular weight: | 681.53 |
| CAS number: | 2133404-55-2 |
| Molecular formula: | C27H13F4N2O11S2- |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
Your cart is empty
$ 
