AF 430 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 10880 | 1 mg | $140 | in stock | |
| 20880 | 5 mg |
$450
|
in stock | |
| 40880 | 25 mg |
$1460
|
in stock | |
| 50880 | 50 mg |
$1890
|
in stock | |
| 60880 | 100 mg |
$2640
|
in stock |
AF 430 is a hydrophilic dye of a coumarin nature. The dye is used in flow cytometry.
This maleimide derivative is reactive against thiol groups. Therefore, it allows labeling of many proteins, including those residing on the cell surface.
Absorption and emission spectra of AF 430
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AF 343 (Coumarin)-X-carboxylic acid
AF 343 (Coumarin) carboxylic acid. Extended aminohexanoyl linker increases solubility and provides spatial separation between the fluorophore, and the functional group.Alkyne-PEG4-amine
A bifunctional linker molecule containing both terminal alkyne moiety for click chemistry, and amine group. Contains a long hydrophilic PEG4 arm.sulfo-Cyanine3 NHS ester
Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.General properties
| Appearance: | yellow solid |
| Mass spec M+ increment: | 625.2 |
| Molecular weight: | 726.8 |
| Molecular formula: | C34H45N4F3O8S |
| IUPAC name: | (8-{6-[2-(2,5-Dioxo-1H-pyrrol-1-yl)ethylamino]-6-oxohexyl}-7,7-dimethyl-2-oxo-4-(trifluoromethyl)-1-oxa-8-aza-5,6,7,8-tetrahydroanthr-5-yl)methanesulfonic acid |
| Solubility: | soluble in water, polar organic solvents (DMF, DMSO) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 430 |
| ε, L⋅mol−1⋅cm−1: | 15955 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.23 |
Product citations
- Yoo, W.; Song, Y.W.; Kim, J.; Ahn, J.; Kim, J.; Shin, Y.; Ryu, J.-K.; Kim, K.K. Molecular basis for SOX2-dependent regulation of super-enhancer activity. Nucleic Acids Research, 2023, 51(22), 11999-12019. doi: 10.1093/nar/gkad908
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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