QuDye dsDNA HS Assay Kit

Fluorometry Nucleic acid quantification
Cat. # Quantity Price Lead time
A2102
10 assays
–   1 days
12102
100 assays
$105 $67 1 days
13102
/ includes tubes
100 assays
$125 $79 1 days
52102
500 assays
$290 $147 1 days
53102
/ includes tubes
500 assays
$389 $176 1 days
72102
1000 assays
$499 1 days
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This kit for DNA quantification is optimized for fluorometers featuring high sensitivity (HS) and a measurements range from 10 pg/uL to 100 ng/uL.The kit includes the concentrated QuDye HS assay reagent, dilution buffer, and DNA standards (0 and 10 ng/uL). The assay is selective towards dsDNA (double-stranded DNA) over RNA and is accurate for initial sample concentrations from 10 pg/uL to 100 ng/uL. The experiment is performed at room temperature and the signal is stable for 3 hours. The assay tolerates contaminants like salts, free nucleotides, detergents, or proteins.

This kit allows to simply dilute the reagent and buffer concentrates and start your measurements. The fluorometer instrument is able to read samples of about 200 uL. For sample preparation mix 180-199 uL of the QuDye HS working solution and 20-1 uL of the DNA sample to be quantified.

QuDye dsDNA HS Assay kit, 53102
QuDye dsDNA HS Assay kit, 53102

Ready-to-use kit manual

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General properties

Storage conditions: Store at 4 °С. Warm to RT before use.
MSDS: Download

Kit

Storage time in months: 12

References

  1. Bonanno Ferraro, G.; Bonomo, C.; Brandtner, D.; Mancini, P.; Veneri, C.; Briancesco, R.; Coccia, A.M.; Lucentini, L.; Suffredini, E.; Bongiorno, D.; Musso, N.; Stefani, S.; La Rosa, G. Characterisation of microbial communities and quantification of antibiotic resistance genes in Italian wastewater treatment plants using 16S rRNA sequencing and digital PCR. Science of The Total Environment, 2024, 933, 173217. doi: 10.1016/j.scitotenv.2024.173217
  2. Grumov, D.; Kostarnoy, A.; Gancheva, P.; Kondratev, A. A Simple and Rapid Microscale Method for Isolating Bacterial Lipopolysaccharides. International Journal of Molecular Sciences, 2024, 25(12), 6345. doi: 10.3390/ijms25126345
  3. Wu, Y.; Leyk, S.; Torabi, H.; Höhn, K.; Honecker, B.; Tauler, M. D. P. M.; Cadar, D.; Jacobs, T.; Bruchhaus, I.; Metwally, N. G. Plasmodium Falciparum Infection Reshapes the Human MicroRNA Profiles of Red Blood Cells and Their Extracellular Vesicles. iScience, 2023, 26(7), 107119. doi: 10.1016/j.isci.2023.107119
  4. Frolov, A.V.; Akhmetova, L.A.; Vishnevskaya, M.S.; Kiriukhin, B.A.; Montreuil, O.; Lopes, F.; Tarasov, S.I. Amplicon metagenomics of dung beetles (Coleoptera , Scarabaeidae , Scarabaeinae) as a proxy for lemur ( Primates , Lemuroidea ) studies in Madagascar. ZooKeys, 2023, 1181, 29-39. doi: 10.3897/zookeys.1181.107496
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