Cell Viability and Cytotoxicity Kit, with Calcein AM and EthD-1
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16472 |
100 assays
|
$199.00
|
21 days |
Cell Viability and Cytotoxicity Kit with Calcein AM and EthD-1 is a ready-to-use kit designed for the simultaneous assessment of cell viability and cytotoxicity using dual-fluorescence staining.
The kit contains two complementary probes:
- Calcein AM is a nonpolar, cell-permeant fluorogenic probe. Once inside live cells, it is hydrolyzed by intracellular esterases to produce bright green fluorescent calcein, which is retained in cells with intact membranes. Fluorescence intensity correlates with metabolic activity and the number of viable cells.
- Ethidium homodimer-1 (EthD-1) is a high-affinity nucleic acid—intercalating dye that is impermeant to live cells due to its relatively large molecular size and dicationic nature. In cells with compromised membrane integrity, EthD-1 passively enters the cytoplasm and nucleus, where it intercalates into double-stranded nucleic acids (DNA and RNA), resulting in a strong enhancement of fluorescence. The dye serves as an indicator of dead cells and cells in the late stages of apoptosis.
Upon co-incubation with cell cultures, viable cells exhibit green fluorescence (calcein), whereas dead and apoptotic cells fluoresce red (EthD-1). Minimal spectral overlap enables two-channel analysis without complex spectral compensation.
Customers also purchased with this product
Annexin V AF 488 Apoptosis Detection Kit, with Propidium Iodide
The ready-to-use kit to label apoptotic and necrotic cells with Annexin V-AF 488 conjugate and propidium iodide.Mitochondrial Membrane Potential Apoptosis Kit, with LumiTracker® Mito Red CMXRos & Annexin V-AF 488
The ready-to-use kit for assaying two key indicators of apoptosis: the externalization of phosphatidylserine and alterations in mitochondrial membrane potential.
Add this product to your cart andget free express delivery
Calcein AM, green fluorescent cell viability probe
A cell-permeant green fluorogenic dye for the assessment of the viability of live cells.Ethidium homodimer 1, red fluorescent nucleic acid stain
Ethidium Homodimer 1 (EthD-1) is a high-affinity, red fluorescent nucleic acid dye impermeant to live cells and widely used to identify dead cells in biological research.LUTOX® Green Ready Stain for Flow Cytometry
A ready-to-use LUTOX Green solution for flow cytometry, a cell-impermeant fluorescent nucleic acid dye used to assess the viability of eukaryotic and prokaryotic cells.General properties
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Transportation: at room temperature for up to 3 weeks. Store at -20 °С. Desiccate. |
| MSDS: | Download |
Spectral properties
| Excitation/absorption maximum, nm: | 492 (Calcein AM); 529 (EthD-1) |
| Emission maximum, nm: | 514 (Calcein AM); 613 (EthD-1) |
Kit
| Storage time in months: | 12 |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Short link - lumiprobe.com/sh/k/X
The count of items is incorrect.
Your cart is empty
$ 
