LumiTracker® Lyso Green

Cat. #	Quantity	Price	Lead time
A1201	1 x 50 uL	–	1 days
B1201	10 x 50 uL	$210	1 days
11201 / optimal price per reaction	20 x 50 uL	$340	1 days

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LumiTracker Lyso Green is a dye that can freely permeate through the cell membranes without any additional detergents; it concentrates in organelles with a low pH such as lysosomes. In contrast with 2,4-dinitrophenol (DNP), this dye does not require the use of antibodies for organelle visualization.

The LumiTracker Lyso Green molecule contains boron dipyrromethene (BDP) fragment, which gives it hydrophobic properties and good cell membrane permeability. In addition, this dye contains an amino group that acts as a weak base. At neutral pH, it is not completely protonated, and the dye molecule remains relatively neutral, thus allowing better cell membrane permeability. Getting into lysosomes with an acidic pH, the dye is likely to become protonated and thus remains in the organelle with a low pH.

Living cells are labeled with nanomolar concentrations of LumiTracker Lyso Green, and staining runs within several minutes. LumiTracker Lyso Green can alter lysosomal pH, so longer incubation can result in increased pH, and it is recommended to stain for 3 to 7 min at 37°C (depending on the specific cell line and staining protocol) before visualization using fluorescent microscopy.

LumiTracker Lyso Green has an absorption maximum at 504 nm and a fluorescence maximum at 511 nm. We also offer LumiTracker Lyso Red for organelle visualization in the red channel (absorption and fluorescence maxima at 577 nm and 590 nm, respectively).

LumiTracker Lyso Green is supplied as a 1 mM solution in DMSO.

Recommendations for using the dye:

Working concentration: 50 to 75 nM.*

Staining: 3 to 7 min at 37°C in 1× PBS (just before visualization, as a separate stage during combined staining with other dyes).

*Concentration and/or staining time can be increased depending on the specific cell line.

LysoTracker™ is a registered trademark of Molecular Probes Inc. in some countries.

Product in action

Vital staining of lysosomes of RAW 264.7 mouse macrophage cells with LumiTracker Lyso Green (x1201)

HK-2 (Human Kidney 2) cells stained with lysosome-specific dye LumiTracker Lyso Green (x1201)

General properties

Storage conditions:	Transportation: at RT for up to 3 weeks. Store at -20°С in the dark. Desiccate. Avoid freeze-thaw cycles. Store in single-use aliquots, if possible.
MSDS:	Download

Spectral properties

Excitation/absorption maximum, nm:	504
ε, L⋅mol⁻¹⋅cm⁻¹:	92000
Emission maximum, nm:	511
Fluorescence quantum yield:	0.97

Kit

Storage time in months:

References

Kasza, K.; Elsherbeny, A.; Moloney, C.; Hardie, K.R.; Cámara, M.; Alexander, C.; Gurnani, P. Hybrid Poly( β ‐amino ester) Triblock Copolymers Utilizing a RAFT Polymerization Grafting‐From Methodology. Macromolecular Chemistry and Physics, 2023, 224(24), 2300262. doi: 10.1002/macp.202300262

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.

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