LumiTracker® Lyso Green
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A1201 |
1 x 50 uL
|
– | 1 days | |
| 21201 |
5 x 50 uL
|
$137 | 1 days | |
| B1201 |
10 x 50 uL
|
$210 | 1 days | |
| 11201
/ optimal price per reaction
|
20 x 50 uL
|
$340 | 1 days |
LumiTracker Lyso Green is a dye that can freely permeate through the cell membranes without any additional detergents; it concentrates in organelles with a low pH such as lysosomes. In contrast with 2,4-dinitrophenol (DNP), this dye does not require the use of antibodies for organelle visualization.
The LumiTracker Lyso Green molecule contains boron dipyrromethene (BDP) fragment, which gives it hydrophobic properties and good cell membrane permeability. In addition, this dye contains an amino group that acts as a weak base. At neutral pH, it is not completely protonated, and the dye molecule remains relatively neutral, thus allowing better cell membrane permeability. Getting into lysosomes with an acidic pH, the dye is likely to become protonated and thus remains in the organelle with a low pH.
Living cells are labeled with nanomolar concentrations of LumiTracker Lyso Green, and staining runs within several minutes. LumiTracker Lyso Green can alter lysosomal pH, so longer incubation can result in increased pH, and it is recommended to stain for 3 to 7 min at 37°C (depending on the specific cell line and staining protocol) before visualization using fluorescent microscopy.
LumiTracker Lyso Green has an absorption maximum at 504 nm and a fluorescence maximum at 511 nm. We also offer LumiTracker Lyso Red for organelle visualization in the red channel (absorption and fluorescence maxima at 577 nm and 590 nm, respectively).
LumiTracker Lyso Green is supplied as a 1 mM solution in DMSO.
Recommendations for using the dye:
Working concentration: 50 to 75 nM.*
Staining: 3 to 7 min at 37°C in 1× PBS (just before visualization, as a separate stage during combined staining with other dyes).
*Concentration and/or staining time can be increased depending on the specific cell line.
LysoTracker™ is a registered trademark of Molecular Probes Inc. in some countries.
Product in action
Vital staining of lysosomes of RAW 264.7 mouse macrophage cells with LumiTracker Lyso Green (x1201)
HK-2 (Human Kidney 2) cells stained with lysosome-specific dye LumiTracker Lyso Green (x1201)
Absorption and emission spectra of LumiTracker Lyso Green
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LumiTracker® Lyso Red
Red-fluorescent reagent for labeling and tracking lysosomes and other acidic organelles with low internal pH in live cells. Analog of LysoTracker™ Red DND-99.General properties
| Storage conditions: | Transportation: at RT for up to 3 weeks. Store at -20°С in the dark. Desiccate. Avoid freeze-thaw cycles. Store in single-use aliquots, if possible. |
| MSDS: | Download |
Spectral properties
| Excitation/absorption maximum, nm: | 504 |
| ε, L⋅mol−1⋅cm−1: | 92000 |
| Emission maximum, nm: | 511 |
| Fluorescence quantum yield: | 0.97 |
Kit
| Storage time in months: | 12 |
References
- Kasza, K.; Elsherbeny, A.; Moloney, C.; Hardie, K.R.; Cámara, M.; Alexander, C.; Gurnani, P. Hybrid Poly( β ‐amino ester) Triblock Copolymers Utilizing a RAFT Polymerization Grafting‐From Methodology. Macromolecular Chemistry and Physics, 2023, 224(24), 2300262. doi: 10.1002/macp.202300262
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