PKH26 Cell Membrane Labeling Kit
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 23201 |
100 uL dye, 5x buffer
|
$225 | 1 days | |
| 13201 |
100 uL dye, 1x buffer
|
$290 | 1 days | |
| 43201 |
500 uL dye, 5x buffer
|
$1125
|
1 days | |
| 33201 |
500 uL dye, 1x buffer
|
$1230
|
1 days |
The ready-to-use kit contains all necessary reagents to label cell membranes with PKH26 dye for evaluating cell migration or proliferation.
The kit contains PKH26 cyanine dye with lipophilic groups, which allow rapid and non-covalent incorporation into cell membranes of almost any cells without influence on cell receptors or transmembrane proteins. The cells preserve their biological properties and ability to proliferate, which makes PKH26 an excellent fluorophore for studying plant and animal cells in vivo and in vitro. It can also be used for studying membrane vesicles.
PKH26 is a red fluorescent dye with an emission maximum at 567 nm. It can be used with other dyes in multicolor assays. PKH26 is rather stable, and PKH26-labeled cells can be studied by microscopy or flow cytometry within 100 days after staining.
The number of measurements using the kit can vary depending on the cell count in the sample:
- The kit containing 100 µL of PHK26 allows analyzing 25 samples of 20 million cells each, using the final PKH26 concentration of
2×10-6 M. - The kit containing 500 µL of PHK26 allows analyzing 125 samples of 20 million cells each, using the final PKH26 concentration of
2×10-6 M.
We recommend choosing the necessary cell count and concentration depending on the cell type and study objectives.
Product in action
Live RAW 264.7 cells stained with PKH26 (x3201) cell membrane labeling kit and LUCS 13 (x4010) and Hoechst 33342 (xH010) nuclear stains
In vivo staining of RAW 264.7 mouse immortalized macrophage cell membranes with PKH26 (x3201) dye
Live mesenchymal stromal cells stained with PKH26 (x3201) cell membrane labeling kit, Hoechst 33342 (xH010) nuclear stain, and Calcein AM cell viability marker
The brain of 11 days-old C57Bl6/CBA mouse. Neuronal cell bodies of triangular septal nucleus stained with PKH26 (x3201) cell membrane labeling kit
Assessment of exosome uptake by M2 macrophages using PKH26 dye (x3201)
Absorption and emission spectra of PKH26
Ready-to-use kit manual
Calculator
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The ready-to-use kit to label cell membranes with green fluorescent PKH2 dye for evaluating cell migration or proliferation.YODi-1, green fluorescent nucleic acid stain
YODi-1 is a cell-impermeant green-fluorescent nucleic acid stain. YODi-1 is chemically equivalent to YOYO®-1.LUCS® 13, green fluorescent nucleic acid stain
LUCS 13 is a cell-permeant green fluorescent nucleic acid stain that exhibits bright, green fluorescence upon binding to nucleic acids.General properties
| Storage conditions: | Store at 4 °С. Warm to RT before use. |
| MSDS: | Download |
Spectral properties
| Excitation/absorption maximum, nm: | 551 |
| ε, L⋅mol−1⋅cm−1: | 133200 |
| Emission maximum, nm: | 566 |
| Fluorescence quantum yield: | 0.40 |
Kit
| Storage time in months: | 12 |
References
- Lercher, A.; Cheong, J.-G.; Jiang, C.; Hoffmann, H.-H.; Ashbrook, A.W.; Yin, Y.S.; Quirk, C.; DeGrace, E.J.; Chiriboga, L.; Rosenberg, B.R.; Josefowicz, S.Z.; Rice, C.M. Antiviral innate immune memory in alveolar macrophages following SARS-CoV-2 infection. bioRxiv, 2023, preprint. doi: 10.1101/2023.11.24.568354
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