QuDye® ssDNA Assay Kit

Fluorometry Nucleic acid quantification
Cat. # Quantity Price Lead time
A7102
10 assays
–   21 days
17102
100 assays
$110 21 days
18102
/ includes tubes
100 assays
$130 21 days
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The kit is intended for quantification of single-stranded DNA (ssDNA) with fluorometer. With this kit you can measure concentration of either oligonucleotide or long ssDNA.

The kit includes concentrated QuDye® ssDNA Reagent, dilution buffer, and ssDNA quantitative standards (0 and 20 ng/uL). For quantification of your samples dilute the reagent using the buffer, add samples (sample volume can vary between 1 and 20 uL), and perform fluorescence measurements with a fluorometer. The range of ssDNA concentrations measured is from 50 pg/μL to 200 ng/μL for initial sample (final DNA content in the assay tube after diluting the sample in the dye working solution is 1–200 ng).

QuDye ssDNA Reagent also binds to double-stranded DNA (dsDNA) and RNA, so they should be avoided in the ssDNA sample. Free nucleotides and other minor contaminants such as salts, detergents, solvents, and proteins have non-significant effect on measurement results.

A wide linear dynamic range of QuDye ssDNA Reagent combined with a small sample volume required for measurements (1–20 uL) makes this kit a quick and convenient tool for quantification of single-stranded DNA.

QuDye ssDNA Assay Kit (optimized for Qubit®), 17102
QuDye ssDNA Assay Kit (optimized for Qubit®), 17102

Excitation and emission spectra of QuDye ssDNA Reagent (ssDNA–dye complex)

Excitation and emission spectra of QuDye ssDNA Reagent (ssDNA–dye complex)

Ready-to-use kit manual

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General properties

Storage conditions: Store at +4 °С. Warm to RT before use.
MSDS: Download

Kit

Storage time in months: 12

References

  1. Gryaznova, M.; Burakova, I.; Smirnova, Y.; Morozova, P.; Chirkin, E.; Gureev, A.; Mikhaylov, E.; Korneeva, O.; Syromyatnikov, M. Effect of Probiotic Bacteria on the Gut Microbiome of Mice with Lipopolysaccharide-Induced Inflammation. Microorganisms, 2024, 12(7), 1341. doi: 10.3390/microorganisms12071341
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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