AF 488 DBCO
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 118F0 | 1 mg | $140 | in stock | |
| 218F0 | 5 mg |
$450
|
in stock | |
| 418F0 | 25 mg |
$1460
|
in stock | |
| 518F0 | 50 mg |
$1890
|
in stock | |
| 618F0 | 100 mg |
$2640
|
in stock |
Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.
AF 488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.
AF 488 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.
Absorption and emission spectra of AF 488
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Cyclooctyne derivative of fluorescein (FAM) for copper-free click chemistry reaction with azides. Pure 6-isomer of fluorescein.Fluo-4 AM, green fluorescent calcium indicator
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| Appearance: | orange solid |
| Molecular weight: | 936.08 |
| Molecular formula: | C48H49N5O11S2 |
| Solubility: | water, DMSO, DMF, methanol |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
Product citations
- Xu, W.; He, M.; Lu, Q. Fibronectin Connecting Cell Sheet Based on Click Chemistry for Wound Repair. Advanced Science, 2024, 11(11), 2306746. doi: 10.1002/advs.202306746
- Xia, Q.; Perera, H. A.; Bolarinho, R.; Piskulich, Z. A.; Guo, Z.; Yin, J.; He, H.; Li, M.; Ge, X.; Cui, Q.; Ramström, O.; Yan, M.; Cheng, J.-X. Click-Free Imaging of Carbohydrate Trafficking in Live Cells Using an Azido Photothermal Probe. Science Advances, 2024, 10(34), eadq0294. doi: 10.1126/sciadv.adq0294
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