AF 647 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16880 | 1 mg | $140 | in stock | |
| 26880 | 5 mg |
$450
|
in stock | |
| 46880 | 25 mg |
$1460
|
in stock | |
| 56880 | 50 mg |
$1890
|
in stock | |
| 66880 | 100 mg |
$2990
|
in stock |
AF 647 maleimide is a thiol-reactive dye for labeling of protein SH groups, and it can be used to attach AF 647 fluorophore to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or with another appropriate reductant prior to the labeling.
AF 647 is a bright, far-red-emitting fluorescent dye with high fluorescence quantum yield and photostability. AF 647 is a water-soluble, pH-insensitive dye. The spectrum of AF 647 is far from the green-yellow wavelengths, which makes this fluorophore indispensable for the microscopy of tissues with high autofluorescence.
Absorption and emission spectra of AF 647
Recommended protocol
Calculator
Customers also purchased with this product
AF 532 NHS ester
NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.FAM phosphoramidite, 6-isomer
FAM (fluorescein) phosphoramidite 6-isomer, is a widely used reagent in nucleic acid chemistry for labeling oligonucleotides. It is derived from the single pure isomer of 6-carboxyfluorescein and separated from amidite linkage via C6 aminohexanol spacer.Cyanine3B DBCO
Dibenzocyclooctyne (DBCO, ADIBO) containing Cyanine3B fluorophore for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 1081.40 |
| CAS number: | 1825332-75-9 |
| Molecular formula: | C41H47K3N4O15S4 |
| Solubility: | good in DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (90+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 655 |
| ε, L⋅mol−1⋅cm−1: | 191800 |
| Emission maximum, nm: | 680 |
| Fluorescence quantum yield: | 0.15 |
| CF260: | 0.09 |
| CF280: | 0.08 |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Short link - lumiprobe.com/sh/p/ab
The count of items is incorrect.
Your cart is empty
$ 
