AF 430 NHS ester

Cat. # Quantity Price Lead time
10820 1 mg $110 in stock
20820 5 mg $165 in stock
40820 25 mg $380 in stock
50820 50 mg $695 in stock
60820 100 mg $1190 in stock
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AF 430 is a hydrophilic dye. It is used in flow cytometry and other applications.

The NHS ester functional group allows to conjugate the fluorophore efficiently with primary and secondary amine groups in both aqueous buffers and anhydrous conditions.

Absorption and emission spectra of AF 430

Absorption and emission spectra of AF 430

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Bifunctional reagent containing alkyne group and hydrazide function. It reacts with aldehydes and ketones. Alkyne group can then be coupled with various azides via CuAAC reaction.

General properties

Appearance: yellow powder
Molecular weight: 701.75
CAS number: 467233-94-9
Molecular formula: C32H42N3F3O9S
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23
CF260: 0,59
CF280: 0,24
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