AF 488 TCO

AF 488 Copper-free click chemistry Cycloalkenes

Cat. #	Quantity	Price	Lead time
118H0	1 mg	$199	in stock
218H0	5 mg	$649	in stock
418H0	25 mg	$1789	in stock

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Trans-cyclooctene (TCO) is one of the most used cycloalkenes for bioconjugation tasks. TCO readily reacts with tetrazines via inverse electron-demand Diels-Alder cycloaddition (IEDDA). TCO-Tetrazine ligation possesses ultrafast kinetics, selectivity, and long-term aqueous stability, which is important in low-concentration applications such as protein-protein conjugations, etc.

This TCO is a AF 488-containing derivative. AF 488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.

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Absorption and emission spectra of AF 488

General properties

Appearance:	orange powder
Molecular weight:	989.27
Molecular formula:	C₄₈H₇₀N₆O₁₂S₂
Solubility:	good in water, DMSO; moderate in acetonitrile
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	495
ε, L⋅mol⁻¹⋅cm⁻¹:	71800
Emission maximum, nm:	519
Fluorescence quantum yield:	0.91
CF₂₆₀:	0.16
CF₂₈₀:	0.10

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.