BAPTA AM, calcium chelator
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
1891-5mg | 5 mg | – | in stock | |
1891-25mg | 25 mg | $149 | in stock |
BAPTA AM is a membrane-permeable form of BAPTA, a highly selective calcium chelator. After loading into a cell, BAPTA AM is converted into BAPTA by cytoplasmic esterases. It is widely used for controlling the cytosolic calcium concentration (Kd = 0.11 μM), inhibiting voltage-gated potassium (Kv) channels, and other applications evaluating the role of intracellular calcium in cell signaling.
BAPTA AM can also be used as a calcium indicator since its absorption maximum changes after complexing with calcium (absorption max. of free/complexed is at 254/274 nm, emission max. of free/complexed is at 363/363 nm).
The commonly used concentration of BAPTA AM for cell signaling studies is 10-100 μM. Because AM esters have low water solubility, mild detergent (such as Pluronic® F-127) is necessary to facilitate loading into the cell.
Absorption and emission spectra of the Calcium ion-BAPTA complex
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Appearance: | white to beige solid |
Molecular weight: | 764.69 |
CAS number: | 126150-97-8 |
Molecular formula: | C34H40N2O18 |
IUPAC name: | N,N'-[1,2-ethanediylbis(oxy-2,1-phenylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-1,1'-bis[(acetyloxy)methyl] ester-glycine |
Solubility: | DMSO |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 274 (complex) |
Emission maximum, nm: | 372 |