BDP® FL ceramide

Golgi apparatus Flow cytometry Microscopy

Cat. #	Quantity	Price	Lead time	Buy this product
3057-50ug	50 ug	–	in stock
3057-250ug	250 ug	$275 $149	in stock

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Ceramides are precursors of sphingolipids composed of sphingosine and a fatty acid joined by an amide bond. This BDP FL ceramide is a synthetic fluorescent lipid, a conjugate of green-emitting BDP FL fluorophore with sphingosine. Inside the cell, BDP FL ceramide is incorporated into the membranes of the Golgi apparatus, so this stain is widely used in cell biology to visualize the Golgi apparatus in living and fixed cells with fluorescence microscopy.

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Live RAW264.7 cells stained with the Golgi apparatus stain BDP FL ceramide (5 μM, 3057-x) and nuclear stain Hoechst 33342.

Absorption and emission spectra of BDP FL

Recommended protocol

Staining the Golgi Apparatus with BDP®-Labeled Ceramides

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General properties

Appearance:	orange semisolid
Molecular weight:	573.57
CAS number:	2730893-01-1
Molecular formula:	C₃₂H₅₀BF₂N₃O₃
Solubility:	good in many organic solvents
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	503
ε, L⋅mol⁻¹⋅cm⁻¹:	92000
Emission maximum, nm:	509
Fluorescence quantum yield:	0.97
CF₂₆₀:	0.015
CF₂₈₀:	0.027

BDP® is a trademark of Lumiprobe

Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.