BDP® FL hydrazide

Cat. # Quantity Price Lead time
11470 1 mg $125 in stock
21470 5 mg $260 in stock
41470 25 mg $510 in stock
51470 50 mg $895 in stock
61470 100 mg $1490 in stock
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BDP FL is a bright and photostable dye for FAM channel. It possesses great photostability, quantum yield approaching unity. This dye is well-suited for microscopy and fluorescence polarization assays.

Hydrazide group provides easy conjugation with carbonyl compounds (aldehydes, and ketones).

Absorption and emission spectra of BDP FL

Absorption and emission spectra of BDP FL

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FAM hydrazide is a carbonyl reactive fluorescein derivative. Pure 5-isomer. Can label oxidized proteins, saccharides, and many small molecules with carbonyl groups.

General properties

Appearance: brown to orange solid
Molecular weight: 342.58
CAS number: 2183473-45-0
Molecular formula: C14H18BClF2N4O
Solubility: poorly soluble in water (20.2 mM = 6.94 g/L), soluble in polar (DMSO, DMF) organic solvents, methanol, moderately soluble in acetonitrile, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 503
ε, L⋅mol−1⋅cm−1: 92000
Emission maximum, nm: 509
Fluorescence quantum yield: 0.97
CF260: 0.015
CF280: 0.027

Product citations

  1. Nojima, S.; Ishida, S.; Terayama, K.; Matsumoto, K.; Matsui, T.; Tahara, S.; Ohshima, K.; Kiyokawa, H.; Kido, K.; Ukon, K.; Yoshida, S.Y.; Mitani, T.T.; Doki, Y.; Mizushima, T.; Okuno, Y.; Susaki, E.A.; Ueda, H.R.; Morii, E. A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases. Cellular and Molecular Gastroenterology and Hepatology, 2022, 14(4), 905-924. doi: 10.1016/j.jcmgh.2022.07.001
  2. Roloff, A.; Nirmalananthan-Budau, N.; Rühle, B.; Borcherding, H.; Thiele, T.; Schedler, U.; Resch-Genger, U. Quantification of Aldehydes on Polymeric Microbead Surfaces via Catch and Release of Reporter Chromophores. Analytical Chemistry, 2019, 91(14), 8827–8834. doi: 10.1021/acs.analchem.8b05515
  3. Liu, R.; Vairaprakash, P.; Lindsey, J.S. Self-assembly with fluorescence readout in a free base dipyrrin–polymer triggered by metal ion binding in aqueous solution. New Journal of Chemistry, 2019, 43(24), 9711–9724. doi: 10.1039/c9nj01787a
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