DusQ 2 CPG 500
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12960 | 100 mg | – | in stock | |
| 32960 | 500 mg |
$530
|
in stock | |
| 42960 | 1 g |
$990
|
in stock | |
| 52960 | 5 g | please inquire | in stock |
This support is intended for automated synthesis of oligonucleotides with 3’-terminal DusQ 2 quencher. Pore size of 500 Å is recommended for the synthesis of oligonucleotides of up to 50 bases in length.
DusQ 2 is a fluorescence quencher with absorption within the range of 560 to 670 nm. It is ideal for effective FRET quenching of fluorophores with emission in this range. The quencher is also used in hybridization probes with static and combined quenching. Its quenching effectiveness does not depend very much on overlapping of fluorophore and quencher spectra, thus allowing for effective quenching of the broad spectrum of fluorophores, including those with emission in the red and far-red part of the spectrum. Thus, DusQ 2 can be used with such fluorophores (including but not limited to) as Cyanine3, TAMRA, ROX, Cyanine3.5, Quasar® 570, Pulsar® 650, Cyanine5, Quasar® 670, Cyanine5.5, and Quasar® 705.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
Absorption spectrum of DusQ 2
Customers also purchased with this product
BDP® TMR azide
Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.FAM phosphoramidite, 5-isomer
Single isomer 5-FAM phosphoramidite for the synthesis of modified oligonucleotides.DusQ 1 CPG 1000
Controlled pore glass (CPG) 1000 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.
Add this product to your cart andget free express delivery
General properties
| Appearance: | dark blue beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| CF260: | 0.31 |
| CF280: | 0.26 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
Your cart is empty
$ 
