DusQ 2 dT phosphoramidite

Oligonucleotide synthesis DusQ 2 Phosphoramidites
Cat. # Quantity Price Lead time
1451-100mg 100 mg $340 in stock
1451-500mg 500 mg $1390 in stock
1451-1g 1 g $2290 in stock
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DusQ 2 dT phosphoramidite is a modified oligo synthesis reagent bearing completely non-fluorescent quencher nucleotide base typically used to internally label DNA oligonucleotide probe. DusQ 2 has large extinction coefficient, efficiently quench fluorescence for dyes emitting in the far red region, such as Cyanine5.

DusQ 2 is typically used to construct qPCR probes with a quencher moiety. Reagent contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges. DusQ 2 has a absorption spectrum in of red and near-infrared range and can be paired with reporter dyes that emit in the range 560–670 nm, e.g. TAMRA, ROX, Cyanine3, and Cyanine5.

Usage

Coupling: 6 minutes coupling time recommended.

Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).

Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.

DusQ2 dT phosphoramidite, 1451-1g
DusQ2 dT phosphoramidite, 1451-1g
Structure of DusQ2 dT phosphoramidite
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General properties

Appearance: black powder
Molecular weight: 1413.55
Molecular formula: C75H89N12O14P
Quality control: NMR 1H, 31P,HPLC-MS (95%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 552

Oligo synthesis details

Coupling conditions: 6 min coupling time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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