Biotin-11-dUTP
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
1553-250nmol | 250 nmol | $140 | 20 days | |
1553-500nmol | 500 nmol | $235 | in stock | |
1553-1umol | 1 umol | $360 | in stock | |
1553-5umol | 5 umol | please inquire | in stock |
Biotinylated deoxyuridine triphosphate (dUTP) can be used for DNA labeling using various methods, including Nick-translation and 3’-terminal non-radioactive labeling. Generated probes can be used in various hybridization experiments such as Southern blot, Northern blot, dot blot, or FISH. Biotin-labeled probes are detected with horseradish peroxidase-conjugated streptavidin or other biomolecule conjugates binding biotin.
«11» in the compound name indicates the length of the linker between dUTP and biotin; this linker increases the efficiency of labeled dUTP incorporation into DNA and interaction of biotin with specific proteins such as avidin/streptavidin or anti-biotin antibodies.
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ROX reference dye for qPCR
ROX is a passive reference dye for qPCR amplification. The dye can be used to normalize fluorescence intensity of reporter dye in qPCR.Linear Polyacrylamide for Nucleic Acid Precipitation
Nucleic acid coprecipitant. Suitable for PCR and RT-PCR reactions.General properties
Appearance: | colorless solid |
Molecular weight: | 878.54 |
Molecular formula: | C28H40N6Li3O17P3S |
IUPAC name: | ((2R,3S,5R)-5-(2,4-dioxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
Solubility: | soluble in water |
Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles. |
MSDS: | Download |
Product specifications |
Product citations
- Krasikova, A.; Kulikova, T.; Schelkunov, M.; Makarova, N.; Fedotova, A.; Berngardt, V.; Maslova, A.; Fedorov, A. The first chicken oocyte nucleus whole transcriptomic profile defines the spectrum of maternal mRNA and non-coding RNA genes transcribed by the lampbrush chromosomes. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.05.577752
- Krasikova, A.; Kulikova, T.; Rodriguez Ramos, J. S.; Maslova, A. Assignment of the Somatic A/B Compartments to Chromatin Domains in Giant Transcriptionally Active Lampbrush Chromosomes. Epigenetics & Chromatin, 2023, 16(1), 24. doi: 10.1186/s13072-023-00499-2