Biotin-X-NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2551-50mg | 50 mg | – | in stock | |
| 2551-100mg | 100 mg | $117 | in stock | |
| 2551-1g | 1 g |
$450
|
in stock |
Biotin may be conjugated to many proteins while maintaining the biological activity. The biotinylated probe is usually detected by avidin, streptavidin due to their effective binding which is used in various applications such as affinity chromatography, ELISA and Western Blot, fluorescence-activated cell sorting (FACS), intracellular labeling.
Biotin-X-NHS ester is a derivative with C6-spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines. This compound is used to attach biotin to primary amines under alkaline conditions (pH 8-9).
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AF 488 conjugate of streptavidin, a high-affinity biotin-binding protein, for immunofluorescence, western blots, microplate assays, and other applications use.General properties
| Appearance: | white solid |
| Mass spec M+ increment: | 339.2 |
| Molecular weight: | 454.55 |
| CAS number: | 72040-63-2 |
| Molecular formula: | C20H30N4O6S |
| IUPAC name: | (2,5-dioxopyrrolidin-1-yl) 6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoate |
| Solubility: | good in DMSO, moderately soluble in DMF |
| Quality control: | NMR 1H |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Product citations
- Rak, A.; Matyushenko, V.; Prokopenko, P.; Kostromitina, A.; Polyakov, D.; Sokolov, A.; Rudenko, L.; Isakova-Sivak, I. A novel immunofluorescent test system for SARS-CoV-2 detection in infected cells. PLOS ONE, 2024, 19(5), e0304534. doi: 10.1371/journal.pone.0304534
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Short link - lumiprobe.com/sh/p/7s
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