Cyanine3.5 NHS ester

Cyanine3.5 NHS esters Protein labeling
Cat. # Quantity Price Lead time
12020 1 mg $125 in stock
22020 5 mg $260 in stock
42020 25 mg $510 in stock
52020 50 mg $895 in stock
62020 100 mg $1490 in stock
Bulk inquiry
Found better price? Let us know and we will propose the way forward!

Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, DyLight 594.

Cyanine3.5 NHS ester, 12020
Cyanine3.5 NHS ester, 12020
Cyanine3.5 NHS ester, 12020
Structure of Cyanine3.5 NHS ester
Download in
ChemDraw format

Cyanine3.5 NHS ester absorbance and emission spectra

Cyanine3.5 NHS ester absorbance and emission spectra

Customers also purchased with this product

BDP® TMR azide

Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.

AF 343 (Coumarin)-X-NHS ester

AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.

FAM azide, 5-isomer

FAM (fluorescein) azide for сlick chemistry labeling, pure 5-isomer.

General properties

Appearance: dark purple solid
Molecular weight: 741.62
CAS number: 2231670-85-0
Molecular formula: C42H44N3BF4O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 591
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35
CF260: 0.29
CF280: 0.22

Product citations

  1. Son, T.; Kim, M.; Choi, M.; Nam, S.H.; Yoo, A.; Lee, H.; Han, E.H.; Hong, K.S.; Park, H.S. Advancing fluorescence imaging: enhanced control of cyanine dye-doped silica nanoparticles. Journal of Nanobiotechnology, 2024, 22, 347. doi: 10.1186/s12951-024-02638-7
  2. Tang, M.; Chen, B.; Xia, H.; Pan, M.; Zhao, R.; Zhou, J.; Yin, Q.; Wan, F.; Yan, Y.; Fu, C.; Zhong, L.; Zhang, Q.; Wang, Y. pH-gated nanoparticles selectively regulate lysosomal function of tumour-associated macrophages for cancer immunotherapy. Nature Communications, 2023, 14, 5888. doi: 10.1038/s41467-023-41592-0
  3. Yin, Q.; Pan, A.; Chen, B.; Wang, Z.; Tang, M.; Yan, Y.; Wang, Y.; Xia, H.; Chen, W.; Du, H.; Chen, M.; Fu, C.; Wang, Y.; Yuan, X.; Lu, Z.; Zhang, Q.; Wang, Y. Quantitative Imaging of Intracellular Nanoparticle Exposure Enables Prediction of Nanotherapeutic Efficacy. Nat Commun, 2021, 12(1), 2385. doi: 10.1038/s41467-021-22678-z
  4. Lee, S.A.; Biteen, J.S. Spectral Reshaping of Single Dye Molecules Coupled to Single Plasmonic Nanoparticles. Journal of Physical Chemistry Letters, 2019, 10, 5764–5769. doi: 10.1021/acs.jpclett.9b02480
Show more (5)
Cy® is a trademark of Cytiva.
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.