dsSafe Nucleic Acid Gel Staining Solution, 10,000×

PCR and qPCR
Cat. # Quantity Price Lead time
16010 100 uL –   in stock
36010 500 uL $117 in stock
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dsSafe is a fluorescent dye for nucleic acid staining in agarose and polyacrylamide gels. dsSafe is the best and safer alternative to ethidium bromide (EtBr); it was shown to be less toxic, non-mutagenic, and non-carcinogenic.

dsSafe allows nucleic acid visualization with sensitivity similar to that of ethidium bromide. Dye excitation can be performed with both conventional UV transilluminators and blue light transilluminators, with the latter ones being safer for the eyes and nucleic acid sample. When blue light is used for dsSafe excitation, background fluorescence is lower than in the case of ethidium bromide excitation with UV light. Excised DNA fragments for subsequent cloning are not exposed to damaging UV radiation, thus increasing cloning efficacy and reducing the frequency of adverse mutations caused by UV.

The spectrum of the DNA-dye complex has two excitation maxima [in the UV (280 nm) and blue ranges (502 nm)] and one emission maximum in the green range (~530 nm). Available as 10,000× concentrate in DMSO. To stain the gel, add the dye to melted agarose solution* or stain the gel after gel electrophoresis in TAE/TBE buffer for 10–20 minutes.

Key dye advantages:

  • Non-mutagenic, non-carcinogenic, far less toxic dye for DNA and RNA staining
  • High sensitivity identical to that of EtBr
  • Excitation both with UV and blue light
  • Easy to use: the dye can be added to melted agarose solution or the gel can be stained within 10 to 20 minutes after gel electrophoresis completion
  • Can be used for all routine laboratory procedures requiring nucleic acid visualization
  • Does not require special storage or disposal conditions

For nucleic acid gel staining, we also offer dsGreen Gel Staining Solution with higher sensitivity to DNA. However, in contrast with dsSafe, gel staining with dsGreen Gel Staining Solution is recommended only after the completion of gel electrophoresis.

*Similarly to ethidium bromide, dsSafe when added in melted agarose solution can slightly reduce the mobility of nucleic acid sample in the gel.

dsSafe Gel Staining Solution 10,000×, 36010
dsSafe Gel Staining Solution 10,000×, 36010
dsSafe Gel Staining Solution 10,000×, 36010
Plasmid DNA after electrophoresis on a 1% agarose gel stained with dsSafe and ethidium bromide. UV excitation with a maximum at 254 nm, visualization using optical filters: 525/50 (dsSafe) and 575/50 (EtBr).

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General properties

Appearance: orange solution
Quality control: UV-Vis abs
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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