DusQ 1 CPG 500
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11960 | 100 mg | – | in stock | |
| 31960 | 500 mg |
$530
|
in stock | |
| 41960 | 1 g |
$990
|
in stock | |
| 51960 | 5 g | please inquire | in stock |
This modified support with a pore size of 500 Å is intended for the synthesis of oligonucleotides of up to 50 bases in length modified with non-fluorescent DusQ 1 quencher at the 3’ end.
DusQ 1 dark quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It can be used for combined quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, FAM™, JOE™, VIC™, R6G, HEX™, TET™, CAL Fluor™ Gold 540, and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
Absorption spectrum of DusQ 1
Customers also purchased with this product
BDP® TMR azide
Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.DMSO (dimethyl sulfoxide), labeling grade
Pure dimethyl sulfoxide (DMSO) solvent with quality optimized for bioconjugation reactions.DusQ 1 dT phosphoramidite
A phosphoramidite used for DusQ 1 dT labelling of oligonucleotides.
Add this product to your cart andget free express delivery
General properties
| Appearance: | purple beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 522 |
| ε, L⋅mol−1⋅cm−1: | 27300 |
| CF260: | 0.17 |
| CF280: | 0.10 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 70−80 |
Your cart is empty
$ 
