DusQ 21 CPG 500

Cat. # Quantity Price Lead time
3151-100mg 100 mg –   in stock
3151-1g 1 g $990 in stock
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DusQ 21 is a quencher for the dyes in far red region of the spectrum. The dye is a xanthene derivative, unlike DusQ 1 or DusQ 2, that are azo dyes. It has a high extinction coefficient, and provides an outstanding quenching efficiency.

The controlled pore glass solid support allows to synthesize oligonucleotides carrying the dye moiety on 3’-terminus.

We recommend the use of ultramild, phenoxyacetyl protected monomers. As an alternative, post-synthetic labeling using NHS ester of DusQ 21 quencher can be used.

Usage

Coupling: Standard conditions identical to normal nucleobases.

Deprotection: 2 hours at room temperature using concentrated ammonia or potassium carbonate K2CO3 (50 mM solution in methanol).

Absorption spectrum of DusQ 21

Absorption spectrum of DusQ 21

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General properties

Appearance: dark blue beads
Quality control: 1H NMR of bound reagent, coupling testing with HPLC-MS
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 656
ε, L⋅mol−1⋅cm−1: 72800

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−80
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