DusQ 3 CPG 500

DusQ 3 CPG modifier solid supports
Cat. # Quantity Price Lead time
1618-100mg 100 mg –   in stock
1618-1g 1 g $990 in stock
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DusQ 3 is a dark quencher for far red and near infrared fluorophores, especially suitable for Cyanine5.5.

The dye can be attached to 3'-terminus of oligonucleotides by means of automated solid phase synthesis using this support. The dye is sensitive to nucleophilic reagents, and therefore must be carefully deprotected. We recommend the use of ultramild, phenoxyacetyl protected monomers. As an alternative, post-synthetic labeling using NHS ester of the quencher can be used.

DusQ 3 CPG 500, 1618-1G
DusQ 3 CPG 500, 1618-1G
DusQ 3 CPG 500, 1618-1G
Structure of DusQ 3 CPG 500
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Absorption spectrum of DusQ 3

Absorption spectrum of DusQ 3

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Controlled pore glass (CPG) 500 solid support for the synthesis of oligonucleotides with 3’-terminal DusQ 1 quencher. Compatible with standard deblocking conditions.
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R6G azide, 5-isomer

Rhodamine 6G is a xanthene dye that has been used for DNA sequencing and qPCR. This azide derivative is used for the attachment of the dye to DNA and other molecules by means of click chemistry.

Cyanine5.5 dimethyl

Non-functionalized Сyanine5.5 dye derivative with good solubility in organic solvents and bright fluorescence in the far-red spectrum range.

General properties

Appearance: dark blue beads
Quality control: 1H NMR of bound reagent, coupling testing with HPLC-MS
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 657
ε, L⋅mol−1⋅cm−1: 45500

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50-80
Coupling conditions: standard, ultramild phosphoramidites
Cleavage conditions: 2 h / rt, 30% aq. ammonia (ultramild protective groups)
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