FAM phosphoramidite, 6-isomer (hydroxyprolinol)
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 25360 | 250 mg | $180 | in stock | |
| 45360 | 1 g |
$575
|
in stock |
FAM phosphoramidite, 6-isomer (Pro) is a hydroxyprolinol-derived reagent suitable for 5’ and internal labeling. Hydroxyprolinol-based (FAM) phosphoramidite is a modifying reagent for the synthesis of labeled with fluorescein oligonucleotides.
For the purpose of the introduction of fluorescent labels this type of phosphoramidites is obtained with a 6-aminohexanoate linkage as a spacer between the skeleton and the functional group.
This modifying reagent with the core of hydroxyprolinol, also has a dimethoxythrityl protection group making available reversed phase HPLC (RP-HPLC) or reversed-phase cartridge purification.
Recommendations for using the reagent:
Coupling: 6 min.
Deprotection: Standard conditions with 25 % ammonium hydroxide; deprotection time depends on the composition of nucleic acids and their protective groups.
AMA (1:1 mixture of concentrated aqueous ammonium hydroxide / 40 % aqueous methylamine) can be used for 2 hours at room temperature or 10 min at 65 °C.
Absorption and emission spectra of FAM
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General properties
| Appearance: | off-white solid |
| Molecular weight: | 1259.44 |
| Molecular formula: | C72H83N4O14P |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| Emission maximum, nm: | 517 |
Oligo synthesis details
| Coupling conditions: | 6 min coupling time recommended |
| Deprotection conditions: | identical to protected nucleobases |
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